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Last updated: 2017/12/10
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CD247 polyclonal antibody

  • Catalog # : PAB12667
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic peptide of CD247.
  • Immunogen:
  • A synthetic peptide corresponding to extracellular domain of human CD247.
  • Host:
  • Rabbit
  • Reactivity:
  • Human, Mouse, Rat
  • Specificity:
  • This antibody recognizes CD247.
  • Form:
  • Liquid
  • Quality Control Testing:
  • Antibody Reactive Against Synthetic Peptide.
  • Recommend Usage:
  • Western Blot (0.1-1 ug/mL)
    ELISA (0.01-0.1 ug/mL)
    Immunoprecipitation (2-5 ug/mL)
    Immunohistochemistry (2-5 ug/mL)
    Flow cytometry (10-20 ug per 106 cells)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In TBS, pH 7.2 (BSA, 10% Proclin300)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C or lower.
    Aliquot to avoid repeated freezing and thawing. Upon initial thawing, apportion into working aliquots and store at -80°C for up to six months.
  • Applications
  • Western Blot
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemical staining of human tonsil tissue stained with CD247 polyclonal antibody (Cat # PAB12667) at 1 : 100 for 10 min at RT. Staining of formalin-fixed tissue requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0 for 10 min followed by colling at RT for 20 min.
  • Immunoprecipitation
  • ELISA
  • Flow Cytometry
  • Application Image
  • Western Blot
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • Immunoprecipitation
  • ELISA
  • Flow Cytometry
  • Gene Information
  • Entrez GeneID:
  • 919
  • Gene Name:
  • CD247
  • Gene Alias:
  • CD3-ZETA,CD3H,CD3Q,CD3Z,T3Z,TCRZ
  • Gene Description:
  • CD247 molecule
  • Gene Summary:
  • The protein encoded by this gene is T-cell receptor zeta, which together with T-cell receptor alpha/beta and gamma/delta heterodimers, and with CD3-gamma, -delta and -epsilon, forms the T-cell receptor-CD3 complex. The zeta chain plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. Low expression of the antigen results in impaired immune response. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq
  • Other Designations:
  • CD247 antigen, zeta subunit,CD3Z antigen, zeta polypeptide (TiT3 complex),OTTHUMP00000032544,T-cell antigen receptor complex, zeta subunit of CD3,T-cell receptor T3 zeta chain,T-cell receptor zeta chain,T-cell surface glycoprotein CD3 zeta chain
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