MS4A1 polyclonal antibody
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More Files
- More Functions
Western Blot (Tissue lysate)
The tissue lysates derived from human spleen were immunoblotted by MS4A1 polyclonal antibody (Cat # PAB12666) at 1 : 1000.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human tonsil tissue with MS4A1 polyclonal antibody (Cat # PAB12666) at 1 : 200 for 10 min at RT. Staining of formalin-fixed tissue requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of MS4A1.
Immunogen
A synthetic peptide corresponding to C-terminus of human MS4A1.
Host
Rabbit
Theoretical MW (kDa)
34
Reactivity
Human
Specificity
This antibody recognizes ~34 KDa of human MS4A1.
Form
Liquid
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
Western Blot (0.1-1 ug/mL)
ELISA (0.01-0.1 ug/mL)
Immunoprecipitation (2-5 ug/mL)
Immunohistochemistry (0.5-2 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In TBS, pH 7.2 (BSA, 10% Proclin300)
Storage Instruction
Store at 4°C. For long term storage store at -20°C or lower.
Aliquot to avoid repeated freezing and thawing. -
Applications
Western Blot (Tissue lysate)
The tissue lysates derived from human spleen were immunoblotted by MS4A1 polyclonal antibody (Cat # PAB12666) at 1 : 1000.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human tonsil tissue with MS4A1 polyclonal antibody (Cat # PAB12666) at 1 : 200 for 10 min at RT. Staining of formalin-fixed tissue requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.Immunoprecipitation
Enzyme-linked Immunoabsorbent Assay
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Gene Info — MS4A1
Entrez GeneID
931Gene Name
MS4A1
Gene Alias
B1, Bp35, CD20, LEU-16, MGC3969, MS4A2, S7
Gene Description
membrane-spanning 4-domains, subfamily A, member 1
Omim ID
112210Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the membrane-spanning 4A gene family. Members of this nascent protein family are characterized by common structural features and similar intron/exon splice boundaries and display unique expression patterns among hematopoietic cells and nonlymphoid tissues. This gene encodes a B-lymphocyte surface molecule which plays a role in the development and differentiation of B-cells into plasma cells. This family member is localized to 11q12, among a cluster of family members. Alternative splicing of this gene results in two transcript variants which encode the same protein. [provided by RefSeq
Other Designations
B-lymphocyte cell-surface antigen B1|CD20 antigen|CD20 receptor
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Interactome
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Pathway
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Disease
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Publication Reference
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Reduced viral burden in paralytic compared to furious canine rabies is associated with prominent inflammation at the brainstem level.
Shuangshoti S, Thepa N, Phukpattaranont P, Jittmittraphap A, Intarut N, Tepsumethanon V, Wacharapluesadee S, Thorner PS, Hemachudha T.
BMC veterinary research 2013 Feb; 9(1):31.
Application：IHC, Dog, Dog spinal cord, Dog brain.
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Rituximab (monoclonal anti-CD20 antibody): mechanisms of action and resistance.
Smith MR.
Oncogene 2003 Oct; 22(47):7359.
Application：Flow Cyt, Human, Mammalian cells.
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Reduced viral burden in paralytic compared to furious canine rabies is associated with prominent inflammation at the brainstem level.
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