PROM1 polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of PROM1.
Immunogen
A synthetic peptide corresponding to 18 amino acids at C-terminus of human PROM1.
Host
Rabbit
Theoretical MW (kDa)
97
Reactivity
Human, Mouse, Rat
Specificity
This antibody recognizes ~97 KDa of mouse or rat PROM1 from mouse embryonic stem cells lystes by Western blot.
Form
Liquid
Purification
Epitope Affinity Purification
Isotype
IgG
Recommend Usage
Western Blot (0.1-1 ug/mL)
ELISA (0.01-0.1 ug/mL)
Immunoprecipitation (2-5 ug/mL)
Immunohistochemistry (2-5 ug/mL)
Flow cytometry (10-20 ug per 106 cells)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (BSA, 10% Proclin300)
Storage Instruction
Store at -20 to -80°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Western Blot (Tissue lysate)
The tissue lysate derived from mouse kidney was immunoprobed by PROM1 polyclonal antibody (Cat # PAB12663) at 1 : 500. An immunoreactive band is observed around ~ 117kDa. This band is abolished by pre-incubation with immunizing peptide.Immunohistochemistry
Immunohistochemical staining of human kidney (A) and human colon (B) tissue sections with PROM1 polyclonal antibody (Cat # PAB12663).Immunoprecipitation
Enzyme-linked Immunoabsorbent Assay
Flow Cytometry
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Gene Info — PROM1
Entrez GeneID
8842Gene Name
PROM1
Gene Alias
AC133, CD133, MSTP061, PROML1, RP41
Gene Description
prominin 1
Omim ID
604365Gene Ontology
HyperlinkGene Summary
This gene encodes a pentaspan transmembrane glycoprotein. The protein localizes to membrane protrusions and is often expressed on adult stem cells, where it is thought to function in maintaining stem cell properties by suppressing differentiation. Mutations in this gene have been shown to result in retinitis pigmentosa and Stargardt disease. Expression of this gene is also associated with several types of cancer. This gene is expressed from at least five alternative promoters that are expressed in a tissue-dependent manner. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
Other Designations
hProminin|hematopoietic stem cell antigen|prominin-like 1
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Interactome
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Disease
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Publication Reference
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Nrf2 deficiency causes hepatocyte dedifferentiation and reduced albumin production in an experimental extrahepatic cholestasis model.
Guo-Ying Wang, Veronica Garcia, Joonyong Lee, Jennifer Yanum, Jingmei Lin, Huaizhou Jiang, Guoli Dai.
PLoS One 2022 Jun; 17(6):e0269383.
Application:WB, Mouse, Liver tissue.
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Evaluation of immunohistochemical expression of stem cell markers (NANOG and CD133) in normal, hyperplastic, and malignant endometrium.
Methaq Al-Kaabi, Khalida Noel, Abdal-Jabbar Al-Rubai.
Journal of Medicine and Life 2022 Jan; 15(1):117.
Application:IHC-P, Human, Human endometrium.
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Pentapartite fractionation of particles in oral fluids by differential centrifugation.
Chiho Hiraga, Satoshi Yamamoto, Sadamitsu Hashimoto, Masataka Kasahara, Tamiko Minamisawa, Sachiko Matsumura, Akira Katakura, Yasutomo Yajima, Takeshi Nomura, Kiyotaka Shiba.
Scientific Reports 2021 Feb; 11(1):3326.
Application:WB, Human, Human pentapartite oral fluid.
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Potentiation of TRAIL-induced cell death by nonsteroidal anti-inflammatory drug in human hepatocellular carcinoma cells through the ER stress-dependent autophagy pathway.
Su-Hoon Lee, Hyun-Jung Moon, Young-Shin Lee, Chi-Dug Kang, Sun-Hee Kim.
Oncology Reports 2020 Sep; 44(3):1136.
Application:WB, Human, SUN-475 cells.
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Crosstalk Between Androgen and Wnt/β-catenin Leads to Changes of Wool Density in FGF5-knockout Sheep.
Rui Zhang, Yan Li, Kun Jia, Xueling Xu, Yao Li, Yue Zhao, Xiaosheng Zhang, Jinlong Zhang, Guoshi Liu, Shoulong Deng, Zhengxing Lian.
Cell Death & Disease 2020 May; 11(5):407.
Application:IF, Sheep, Dermal papilla cells.
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CD147 Promotes Cell Small Extracellular Vesicles Release during Colon Cancer Stem Cells Differentiation and Triggers Cellular Changes in Recipient Cells.
Lucchetti D, Colella F, Perelli L, Ricciardi-Tenore C, Calapà F, Fiori ME, Carbone F, De Maria R, Sgambato A.
Cancers 2020 Jan; 12(2):E260.
Application:WB-Ce, Human, CSC2, HT-2, HT-29 cells.
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Endothelial progenitor cell transplantation alleviated ischemic brain injury via inhibiting C3/C3aR pathway in mice.
Yuanyuan Ma, Lu Jiang, Liping Wang, Yongfang Li, Yanqun Liu, Wenjing Lu, Rubing Shi, Linyuan Zhang, Zongjie Fu, Meijie Qu, Yingling Liu, Yongting Wang, Zhijun Zhang, Guo-Yuan Yang.
Journal of Cerebral Blood Flow and Metabolism 2019 Dec; 271678X19892777.
Application:IF, Human, Endothelial progenitor cell.
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Cancer-associated fibroblasts in the tumor microenvironment of tongue carcinoma is a heterogeneous cell population.
Vered M, Shnaiderman-Shapiro A, Zlotogorski-Hurvitz A, Salo T, Yahalom R.
Acta Histochemica 2019 Nov; 121(8):151446.
Application:IHC, Human, Human tongue squamous cell carcinoma.
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Expression of CD133 is associated with poor prognosis in stage II colorectal carcinoma.
Park YY, An CH, Oh ST, Chang ED, Lee J.
Medicine 2019 Aug; 98(32):e16709.
Application:IHC-P, Human, Human colorectal carcinoma.
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Expansion of Luminal Progenitor Cells in the Aging Mouse and Human Prostate.
Crowell PD, Fox JJ, Hashimoto T, Diaz JA, Navarro HI, Henry GH, Feldmar BA, Lowe MG, Garcia AJ, Wu YE, Sajed DP, Strand DW, Goldstein AS.
Cell Reports 2019 Aug; 28(6):1499.
Application:WB, Mouse, Mouse basal-derived organoids.
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Combined prognostic value of the cancer stem cell markers CD47 and CD133 in esophageal squamous cell carcinoma.
Wang JH, Huang ST, Zhang L, Liu ZG, Liang RX, Jiang SW, Jiang YN, Yu XJ, Jiang YC, Li XZ, Zhang PF, Wen ZS, Zheng M.
Cancer Medicine 2019 Mar; 8(3):1315.
Application:IHC, Human, Esophageal squamous cell carcinoma.
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miR-142-3p is a tumor suppressor that inhibits estrogen receptor expression in ER-positive breast cancer.
Behzad Mansoori, Ali Mohammadi, Morten F Gjerstorff, Solmaz Shirjang, Zahra Asadzadeh, Vahid Khaze, Uffe Holmskov, Tohid Kazemi, Pascal H G Duijf, Behzad Baradaran.
Journal of Cellular Physiology 2019 Sep; 234(9):16043.
Application:WB-Tr, Human, MCF-7 cells.
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Identification of novel PROM1 mutations responsible for autosomal recessive maculopathy with rod-cone dystrophy.
Liang J, She X, Chen J, Zhai Y, Liu Y, Zheng K, Gong Y, Zhu H, Luo X, Sun X.
Graefe's Archive for Clinical and Experimental Ophthalmology 2019 Mar; 257(3):619.
Application:IF, WB-Tr, Human, HEK 293T cells.
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Inhibition of skin carcinogenesis by suppression of NF-κB dependent ITGAV and TIMP-1 expression in IL-32γ overexpressed condition.
Lee YS, Lee CH, Bae JT, Nam KT, Moon DB, Hwang OK, Choi JS, Kim TH, Jun HO, Jung YS, Hwang DY, Han SB, Yoon DY, Hong JT.
Journal of Experimental & Clinical Cancer Research 2018 Nov; 37(1):293.
Application:IHC-P, WB, Mouse, A-431, SK-Mel-28 cells, Human skin tumor, Mouse skin.
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Novel role of O-glycosyltransferases GALNT3 and B3GNT3 in the self-renewal of pancreatic cancer stem cells.
Barkeer S, Chugh S, Karmakar S, Kaushik G, Rauth S, Rachagani S, Batra SK, Ponnusamy MP.
BMC Cancer 2018 Nov; 18(1):1157.
Application:WB, Human, Capan1 cells.
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Low P16INK4A Expression Associated with High Expression of Cancer Stem Cell Markers Predicts Poor Prognosis in Cervical Cancer after Radiotherapy.
Fu HC, Chuang IC, Yang YC, Chuang PC, Lin H, Ou YC, Chang Chien CC, Huang HS, Kang HY.
International Journal of Molecular Sciences 2018 Aug; 19(9):E2541.
Application:WB-Tr, Human, HeLa cells.
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cxcl12 Gene Engineered Endothelial Progenitor Cells Further Improve the Functions of Oligodendrocyte Precursor Cells.
Yuan F, Chang S, Luo L, Li Y, Wang L, Song Y, Qu M, Zhang Z, Yang GY, Wang Y.
Experimental Cell Research 2018 Jun; 367(2):222.
Application:IF, Human, Endothelial progenitor cells.
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Noninvasive evaluation of the migration effect of transplanted endothelial progenitor cells in ischemic muscle using a multimodal imaging agent.
Peng X, Li C, Bai Y, Wang X, Zhang Y, An Y, Teng GJ, Ju S.
International Journal of Nanomedicine 2018 Mar; 13:1819.
Application:IF, Mouse, Mouse endothelial progenitor cells.
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Isoliquiritigenin inhibits the proliferation and induces the differentiation of human glioma stem cells.
Lin Y, Sun H, Dang Y, Li Z.
Oncology Reports 2018 Feb; 39(2):687.
Application:IF, Human, Human glioma stem cells.
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Evidence of renal angiomyolipoma neoplastic stem cells arising from renal epithelial cells.
Filipa Gonçalves A, Adlesic M, Brandt S, Hejhal T, Harlander S, Sommer L, Shakhova O, Wild PJ, Frew IJ.
Nature Communications 2017 Nov; 8(1):1466.
Application:IF, IHC-P, Human, Human renal angiomyolipomas, proximal tubule epithelial cells.
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Hyperglycemia Alters the Protein Levels of Prominin-1 and VEGFA in the Retina of Albino Rats.
Almasry SM, Habib EM, Elmansy RA, Hassan ZA.
The Journal of Histochemistry and Cytochemistry: Official Journal of the Histochemistry Society 2017 Oct; 1:2215541773.
Application:IHC-P, Rat, Rat retinal tissue sections.
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Effect of heparan sulfate mimetics from Escherichia coli K5 polysaccharide on SDF-1/CXCL12-induced endothelial progenitor cells in vitro.
Fu H, Deng C, Teng L, Cai Z, Chen J, Lu G.
International Journal of Biological Macromolecules 2018 Feb; 107(Pt B):2492.
Application:Flow Cyt, Human, Human endothelial progenitor cells.
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Auramine O, an incense smoke ingredient, promotes lung cancer malignancy.
Tung JC, Huang WC, Yang JC, Chen GY, Fan CC, Chien YC, Lin PS, Candice Lung SC, Chang WC.
Environmental Toxicology 2017 Nov; 32(11):2379.
Application:WB-Ce, Human, A-549 cells.
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A subpopulation of cancer stem cells identifies radiographic characteristics in glioblastoma.
Kim JE, Kim SK, Shin J, Se YB, Choi SH, Park SH, Choi SA, Lee JY Phi JH, Wang KC, Park CK, Kim SK.
Oncology Letters 2017 Mar; 13(3):1175.
Application:IHC-P, Human, Human glioblastoma.
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MiR-34a induces apoptosis of glioma stem cells via the inhibition of SIRT1.
Xiaoyong Fan X, Wang J, Cui Y, Liu G.
International journal of Clinical and Experimental Pathology 2016 Oct; 9(10):9715.
Application:IF, Flow Cyt, Human, Glioma, neural glioma stem cell, U87.
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Monoclonal Antibodies 13A4 and AC133 Do Not Recognize the Canine Ortholog of Mouse and Human Stem Cell Antigen Prominin-1 (CD133).
Thamm K, Graupner S, Werner C, Huttner WB, Corbeil D.
PLoS One 2016 Oct; 11(10):e0164079.
Application:Flow Cyt, IF, Dog, MDCK cells.
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Characterization of Ovine Dermal Papilla Cell Aggregation.
Sari AR, Rufaut NW, Jones LN, Sinclair RD.
International Journal of Trichology 2016 Aug; 8(3):121.
Application:IF, Sheep, Sheep dermal papilla cells.
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Prognostic Impact and Clinicopathological Correlation of CD133 and ALDH1 Expression in Invasive Breast Cancer.
Kim SJ, Kim YS, Jang ED, Seo KJ, Kim JS.
Journal of Breast Cancer 2015 Dec; 18(4):347.
Application:IHC-P, Human, Breast cancer.
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Low Intensity Laser Irradiation at 636 nm Induces Increased Viability and Proliferation in Isolated Lung Cancer Stem Cells.
Crous A, Abrahamse H.
Photomedicine and Laser Surgery 2016 Nov; 34(11):525.
Application:IF, Human, Lung cancer stem cells.
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Putative CD133 + melanoma cancer stem cells induce initial angiogenesis in vivo.
Zimmer RM, Matthiesen P, Kreher F, Kampmann A, Spalthoff S, Jehn P, Bittermann G, Gellrich N, Tavassol F.
Microvascular Research 2016 Mar; 104:46.
Application:IHC, Mouse, Dorsal skinfold chamber.
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Profiles of Cancer Stem Cell Subpopulations in Cholangiocarcinomas.
Cardinale V,Renzi A,Carpino G,Torrice A,Bragazzi MC,Giuliante F,DeRose AM,Fraveto A,Onori P,Napoletano C,Franchitto A,Cantafora A,Grazi G,Caporaso N,DArgenio G,Alpini G,Reid LM,Gaudio E,Alvaro D.
The American Journal of Pathology 2015 Jun; 185(6):1724.
Application:IF, IHC-P, Human, Cholangiocarcinoma, CCA cells.
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Decreased Expression of Stem Cell Markers by Simvastatin in 7,12-dimethylbenz(a)anthracene (DMBA)-induced Breast Cancer.
Renno AL, Alves-Junior MJ, Rocha RM, De Souza PC, de Souza VB, Jampietro J, Vassallo J, Hyslop S, Anhe GF, de Moraes Schenka NG, Soares FA, Schenka AA.
Toxicologic Pathology 2015 Apr; 43(3):400.
Application:IHC-P, Rat, Breast cancer.
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Immunohistochemical analysis of cancer stem cell markers in pancreatic adenocarcinoma patients after neoadjuvant chemoradiotherapy.
Mizukami T, Kamachi H, Mitsuhashi T, Tsuruga Y, Hatanaka Y, Kamiyama T, Matsuno Y, Taketomi A.
BMC Cancer 2014 Sep; 14(1):687.
Application:IHC-P, Human, Pancreatic adenocarcinoma.
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Nrf2 Is Involved in Maintaining Hepatocyte Identity during Liver Regeneration.
Zou Y, Lee J, Nambiar SM, Hu M, Rui W, Bao Q, Chan JY, Dai G.
PLoS One 2014 Sep; 9(9):e107423.
Application:WB-Ti, Mouse, Liver.
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Subcellular Localization of CD133 and Interleukin-6 Receptor (IL-6R) in Human Hepatoblastoma Cell-Line (HuH-6 Clone-5).
Akita M, Matsumoto S, Murail N, Komatsu K, Fujita K.
Journal of Stem Cells Research, Development & Therapy 2014 Dec; 1:1.
Application:IF, IHC, EM, Human, Human hepatoblastoma cells (HuH-6 clone 5).
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Insights into the infiltrative behavior of adamantinomatous craniopharyngioma in a new xenotransplant mouse model.
Stache C, Holsken A, Schlaffer SM, Hess A, Metzler M, Frey B, Fahlbusch R, Flitsch J, Buchfelder M, Buslei R.
Brain Pathology 2015 Jan; 25(1):1.
Application:IHC, Mouse, Tumor stem cell.
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Drug-resistant colon cancer cells produce high carcinoembryonic antigen and might not be cancer-initiating cells.
Lee HC, Ling QD, Yu WC, Hung CM, Kao TC, Huang YW, Higuchi A.
Drug Design, Development and Therapy 2013 Jun; 7:491.
Application:Flow Cyt, IS, Human, LoVo cells.
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A microRNA-135a/b binding polymorphism in CD133 confers decreased risk and favorable prognosis of lung cancer in Chinese by reducing CD133 expression.
Cheng M, Yang L, Yang R, Yang X, Deng J, Yu B, Huang D, Zhang S, Wang H, Qiu F, Zhou Y, Lu J.
Carcinogenesis 2013 Oct; 34(10):2292.
Application:IHC, WB-Ti, Human, Lung cancer.
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Identification and characterization of a novel scFv recognizing human and mouse CD133.
Swaminathan SK, Niu L, Waldron N, Kalscheuer S, Zellmer DM, Olin MR, Ohlfest JR, Vallera DA, Panyam J.
Drug Delivery and Translational Research 2013 Apr; 3(2):143.
Application:Flow Cyt, WB-Ce, Human, Mouse, 4T1, Caco-2, NIH-3T3, ONC49M2, U87 cells.
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CD133, a stem cell marker in mice: results in animal models cannot always be applied to human skin.
Sellheyer K, Nelson P.
Journal of Cutaneous Pathology 2013 Jul; 40(7):690.
Application:IHC, Human, Mouse, Kidney, Skin.
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Expression of LGR-5, MSI-1 and DCAMKL-1, putative stem cell markers, in the early phases of 1,2-dimethylhydrazine-induced rat colon carcinogenesis: correlation with nuclear β-catenin.
Femia AP, Dolara P, Salvadori M, Caderni G.
BMC Cancer 2013 Feb; 13(48).
Application:IHC, Rat, Rat colon.
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The therapeutic effect of vascular endothelial growth factor gene- or heme oxygenase-1 gene-modified endothelial progenitor cells on neovascularization of rat hindlimb ischemia model.
Long J, Wang S, Zhang Y, Liu X, Zhang H, Wang S.
Journal of Vascular Surgery 2013 Apr; 58(3):756.
Application:Flow Cyt, Rat, Endothelial progenitor cells.
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Evaluation of expression profiles of hematopoietic stem cell, endothelial cell, and myeloid cell antigens in spontaneous and chemically induced hemangiosarcomas and hemangiomas in mice.
Kakiuchi-Kiyota S, Crabbs TA, Arnold LL, Pennington KL, Cook JC, Malarkey DE, Cohen SM.
Toxicologic Pathology 2013 Jul; 41(5):709.
Application:IHC-P, Mouse, Liver, Stomach, Adipose tissue, Uterus, Spleen, Subcutaneous, Heart, Intestine, Intestine, Pancreas, Skin.
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Branched-chain amino acid-enriched nutrients stimulate antioxidant DNA repair in a rat model of liver injury induced by carbon tetrachloride.
Ichikawa K, Okabayashi T, Shima Y, Iiyama T, Takezaki Y, Munekage M, Namikawa T, Sugimoto T, Kobayashi M, Mimura T, Hanazaki K.
Molecular Biology Reports 2012 Dec; 39(12):10803.
Application:WB-Ti, Rat, Rat livers.
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Nrf2 deficiency causes hepatocyte dedifferentiation and reduced albumin production in an experimental extrahepatic cholestasis model.
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