Rabbit polyclonal antibody raised against synthetic peptide of PROM1.
A synthetic peptide corresponding to 18 amino acids at C-terminus of human PROM1.
Theoretical MW (kDa):
Human, Mouse, Rat
This antibody recognizes ~97 KDa of mouse or rat PROM1 from mouse embryonic stem cells lystes by Western blot.
Epitope Affinity Purification
Western Blot (0.1-1 ug/mL) ELISA (0.01-0.1 ug/mL) Immunoprecipitation (2-5 ug/mL) Immunohistochemistry (2-5 ug/mL) Flow cytometry (10-20 ug per 106 cells) The optimal working dilution should be determined by the end user.
In PBS, pH 7.2 (BSA, 10% Proclin300)
Store at -20 to -80°C. Aliquot to avoid repeated freezing and thawing.
The tissue lysate derived from mouse kidney was immunoprobed by PROM1 polyclonal antibody (Cat # PAB12663) at 1 : 500. An immunoreactive band is observed around ~ 117kDa. This band is abolished by pre-incubation with immunizing peptide.
Immunohistochemical staining of human kidney (A) and human colon (B) tissue sections with PROM1 polyclonal antibody (Cat # PAB12663).
This gene encodes a pentaspan transmembrane glycoprotein. The protein localizes to membrane protrusions and is often expressed on adult stem cells, where it is thought to function in maintaining stem cell properties by suppressing differentiation. Mutations in this gene have been shown to result in retinitis pigmentosa and Stargardt disease. Expression of this gene is also associated with several types of cancer. This gene is expressed from at least five alternative promoters that are expressed in a tissue-dependent manner. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq