Mycobacterium tuberculosis EsaT-6 polyclonal antibody
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More Files
- More Functions
Western Blot
Western blotting analysis (reducing conditions) of recombinant protein EsaT - 6 using EsaT - 6 polyclonal antibody (Cat # PAB1266). Lane 1 , 3 , 5 : Cell lysates of non - transfected E. coli (negative controls). Lane 2 , 4 , 6 : Cell lysate of EsaT - 6 - transfected E. coli; dilution (2) 1 ug/mL , (4) 0.2 ug/mL , (6) 0.1 ug/mL ; detection with Donkey anti - rabbit / HRP secondary antibody.
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Specification
Product Description
Rabbit polyclonal antibody raised against Mycobacterium tuberculosis EsaT-6 (Rv3875).
Immunogen
ESAT-6 (Rv3875) of Mycobacterium tuberculosis.
Host
Rabbit
Theoretical MW (kDa)
11.8
Reactivity
Bacteria
Specificity
This antibody reacts with EsaT-6, a 11.8 KDa antigen encoded by gene Rv3875 of Mycobacterium tuberculosis.
This antibody recognizes EsaT-6 of Mycobacterium tuberculosis H37Rv and Mycobacterium bovis; it reacts also with recombinant antigen produced in Escherichia coli.Form
Liquid
Recommend Usage
The optimal working dilution should be determined by the end user.
Storage Buffer
In PBS, pH 7.4 (0.09% sodium azide)
Storage Instruction
Store at 4°C. Do not freeze.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blotting analysis (reducing conditions) of recombinant protein EsaT - 6 using EsaT - 6 polyclonal antibody (Cat # PAB1266). Lane 1 , 3 , 5 : Cell lysates of non - transfected E. coli (negative controls). Lane 2 , 4 , 6 : Cell lysate of EsaT - 6 - transfected E. coli; dilution (2) 1 ug/mL , (4) 0.2 ug/mL , (6) 0.1 ug/mL ; detection with Donkey anti - rabbit / HRP secondary antibody.
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