Immunohistochemical staining of human prostate cancer tissue stained with AR polyclonal antibody (Cat # PAB12655) at 1 : 100 for 10 min at RT. Staining of formalin-fixed tissue requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.
5 mg of the whole cell lysate derived from conditioned LNCaP were immunoprecipitated by 4 ug of Phospho-AR S210 polyclonal antibody (Cat # PAB12654, lane 1) or pre adsorbed by immunization peptide (lane 2) followed by AR polyclonal antibody (Cat # PAB12655) at 1 : 500.
The androgen receptor gene is more than 90 kb long and codes for a protein that has 3 major functional domains: the N-terminal domain, DNA-binding domain, and androgen-binding domain. The protein functions as a steroid-hormone activated transcription factor. Upon binding the hormone ligand, the receptor dissociates from accessory proteins, translocates into the nucleus, dimerizes, and then stimulates transcription of androgen responsive genes. This gene contains 2 polymorphic trinucleotide repeat segments that encode polyglutamine and polyglycine tracts in the N-terminal transactivation domain of its protein. Expansion of the polyglutamine tract causes spinal bulbar muscular atrophy (Kennedy disease). Mutations in this gene are also associated with complete androgen insensitivity (CAIS). Two alternatively spliced variants encoding distinct isoforms have been described. [provided by RefSeq