AR (phospho S210) polyclonal antibody
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More Files
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Immunoprecipitation
5 mg of the whole cell lysate derived from conditioned LNCaP were immunoprecipitated by 4 ug of AR (phospho S210) polyclonal antibody (Cat # PAB12654, lane 1) or pre adsorbed by immunization peptide (lane 2) followed by AR polyclonal antibody (Cat # PAB12655) at 1 : 500.
Dot Blot (Peptide)
Dot Blot : 1 ug peptide was blot onto NC membrane.
A : AR (pS210).
B : AR (Non-phospho).
Followed were blotted at a 1 : 2000 dilution by AR (phospho S210) polyclonal antibody (Cat # PAB12654).
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic phosphopeptide of AR.
Immunogen
Synthetic phosphopeptide corresponding to residues surrounding S210 of human AR.
Sequence
EASGA
Host
Rabbit
Theoretical MW (kDa)
110
Reactivity
Human, Mouse, Rat
Specificity
This antibody recognizes ~110 KDa of human AR.
Form
Liquid
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
Western Blot (0.1-1 ug/mL)
ELISA (0.01-0.1 ug/mL)
Immunoprecipitation (2-5 ug/mL)
Immunohistochemistry (2-5 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In TBS, pH 7.2 (BSA, 10% Proclin300)
Storage Instruction
Store at -20°C. For long term storage store at -80°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Western Blot
Immunohistochemistry
Immunoprecipitation
5 mg of the whole cell lysate derived from conditioned LNCaP were immunoprecipitated by 4 ug of AR (phospho S210) polyclonal antibody (Cat # PAB12654, lane 1) or pre adsorbed by immunization peptide (lane 2) followed by AR polyclonal antibody (Cat # PAB12655) at 1 : 500.Enzyme-linked Immunoabsorbent Assay
Dot Blot (Peptide)
Dot Blot : 1 ug peptide was blot onto NC membrane.
A : AR (pS210).
B : AR (Non-phospho).
Followed were blotted at a 1 : 2000 dilution by AR (phospho S210) polyclonal antibody (Cat # PAB12654). -
Gene Info — AR
Entrez GeneID
367Gene Name
AR
Gene Alias
AIS, DHTR, HUMARA, KD, NR3C4, SBMA, SMAX1, TFM
Gene Description
androgen receptor
Gene Ontology
HyperlinkGene Summary
The androgen receptor gene is more than 90 kb long and codes for a protein that has 3 major functional domains: the N-terminal domain, DNA-binding domain, and androgen-binding domain. The protein functions as a steroid-hormone activated transcription factor. Upon binding the hormone ligand, the receptor dissociates from accessory proteins, translocates into the nucleus, dimerizes, and then stimulates transcription of androgen responsive genes. This gene contains 2 polymorphic trinucleotide repeat segments that encode polyglutamine and polyglycine tracts in the N-terminal transactivation domain of its protein. Expansion of the polyglutamine tract causes spinal bulbar muscular atrophy (Kennedy disease). Mutations in this gene are also associated with complete androgen insensitivity (CAIS). Two alternatively spliced variants encoding distinct isoforms have been described. [provided by RefSeq
Other Designations
OTTHUMP00000023450|OTTHUMP00000061928|dihydrotestosterone receptor
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Interactome
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Pathway
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Disease
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Publication Reference
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Androgen receptor gene knockout male mice exhibit impaired cardiac growth and exacerbation of angiotensin II-induced cardiac fibrosis.
Ikeda Y, Aihara K, Sato T, Akaike M, Yoshizumi M, Suzaki Y, Izawa Y, Fujimura M, Hashizume S, Kato M, Yagi S, Tamaki T, Kawano H, Matsumoto T, Azuma H, Kato S, Matsumoto T.
The Journal of Biological Chemistry 2005 Aug; 280(33):29661.
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Androgen receptor gene knockout male mice exhibit impaired cardiac growth and exacerbation of angiotensin II-induced cardiac fibrosis.
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