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Last updated: 2017/4/23
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SOX9 (phospho S181) polyclonal antibody

  • Catalog # : PAB12646
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic phosphopeptide of SOX9.
  • Immunogen:
  • Synthetic phosphopeptide corresponding to residues surrounding S181 of human SOX9.
  • Sequence:
  • RKSVK
  • Host:
  • Rabbit
  • Theoretical MW (kDa):
  • 56
  • Reactivity:
  • Human, Mouse, Rat
  • Specificity:
  • This antibody recognizes ~56 KDa of human SOX9 at the phosphorylation site of Ser181. It does not cross react to non-phospho SOX9.
  • Form:
  • Liquid
  • Quality Control Testing:
  • Antibody Reactive Against Synthetic Peptide.
  • Recommend Usage:
  • Western Blot (0.1-1 ug/mL)
    ELISA (0.01-0.1 ug/mL)
    Immunoprecipitation (2-5 ug/mL)
    Immunohistochemistry (2-5 ug/mL)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In TBS, pH 7.2 (BSA, 10% Proclin300)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C or -80°C.
    Aliquot to avoid repeated freezing and thawing.
  • Publication Reference
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • The whole cell lysate derived from conditioned LNCaP were immunoblotted SOX9 (phospho S181) polyclonal antibody (Cat # PAB12646) at 1 : 500.
  • Immunohistochemistry
  • Immunoprecipitation
  • ELISA
  • Dot Blot (Peptide)
  • Dot Blot (Peptide)
  • Dot Blot : 1 ug peptide was blot onto NC membrane.
    A : SOX9 (pS181).
    B : SOX9 (Paired S181) (Non-phospho).
    C : Non-related phosphospecific phosphopeptide.
    Followed were blotted at a 1:1000 dilution by :
    1 : SOX9 (phospho S181) polyclonal antibody (Cat # PAB12646).
    2 : SOX9 polyclonal antibody (Cat # PAB12737).
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunohistochemistry
  • Immunoprecipitation
  • ELISA
  • Gene Information
  • Entrez GeneID:
  • 6662
  • Gene Name:
  • SOX9
  • Gene Alias:
  • CMD1,CMPD1,SRA1
  • Gene Description:
  • SRY (sex determining region Y)-box 9
  • Gene Summary:
  • The protein encoded by this gene recognizes the sequence CCTTGAG along with other members of the HMG-box class DNA-binding proteins. It acts during chondrocyte differentiation and, with steroidogenic factor 1, regulates transcription of the anti-Muellerian hormone (AMH) gene. Deficiencies lead to the skeletal malformation syndrome campomelic dysplasia, frequently with sex reversal. [provided by RefSeq
  • Other Designations:
  • SRY (sex determining region Y)-box 9 (campomelic dysplasia, autosomal sex-reversal),SRY (sex-determining region Y)-box 9 (campomelic dysplasia, autosomal sex-reversal),SRY (sex-determining region Y)-box 9 protein,campomelic dysplasia, autosomal sex-revers
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