SOX9 (phospho S181) polyclonal antibody
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More Files
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Western Blot (Cell lysate)
The whole cell lysate derived from conditioned LNCaP were immunoblotted SOX9 (phospho S181) polyclonal antibody (Cat # PAB12646) at 1 : 500.
Dot Blot (Peptide)
Dot Blot : 1 ug peptide was blot onto NC membrane.
A : SOX9 (pS181).
B : SOX9 (Paired S181) (Non-phospho).
C : Non-related phosphospecific phosphopeptide.
Followed were blotted at a 1:1000 dilution by :
1 : SOX9 (phospho S181) polyclonal antibody (Cat # PAB12646).
2 : SOX9 polyclonal antibody (Cat # PAB12737).
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic phosphopeptide of SOX9.
Immunogen
Synthetic phosphopeptide corresponding to residues surrounding S181 of human SOX9.
Sequence
RKSVK
Host
Rabbit
Theoretical MW (kDa)
56
Reactivity
Human, Mouse, Rat
Specificity
This antibody recognizes ~56 KDa of human SOX9 at the phosphorylation site of Ser181. It does not cross react to non-phospho SOX9.
Form
Liquid
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
Western Blot (0.1-1 ug/mL)
ELISA (0.01-0.1 ug/mL)
Immunoprecipitation (2-5 ug/mL)
Immunohistochemistry (2-5 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In TBS, pH 7.2 (BSA, 10% Proclin300)
Storage Instruction
Store at 4°C. For long term storage store at -20°C or -80°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Western Blot (Cell lysate)
The whole cell lysate derived from conditioned LNCaP were immunoblotted SOX9 (phospho S181) polyclonal antibody (Cat # PAB12646) at 1 : 500.Immunohistochemistry
Immunoprecipitation
Enzyme-linked Immunoabsorbent Assay
Dot Blot (Peptide)
Dot Blot : 1 ug peptide was blot onto NC membrane.
A : SOX9 (pS181).
B : SOX9 (Paired S181) (Non-phospho).
C : Non-related phosphospecific phosphopeptide.
Followed were blotted at a 1:1000 dilution by :
1 : SOX9 (phospho S181) polyclonal antibody (Cat # PAB12646).
2 : SOX9 polyclonal antibody (Cat # PAB12737). -
Gene Info — SOX9
Entrez GeneID
6662Gene Name
SOX9
Gene Alias
CMD1, CMPD1, SRA1
Gene Description
SRY (sex determining region Y)-box 9
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene recognizes the sequence CCTTGAG along with other members of the HMG-box class DNA-binding proteins. It acts during chondrocyte differentiation and, with steroidogenic factor 1, regulates transcription of the anti-Muellerian hormone (AMH) gene. Deficiencies lead to the skeletal malformation syndrome campomelic dysplasia, frequently with sex reversal. [provided by RefSeq
Other Designations
SRY (sex determining region Y)-box 9 (campomelic dysplasia, autosomal sex-reversal)|SRY (sex-determining region Y)-box 9 (campomelic dysplasia, autosomal sex-reversal)|SRY (sex-determining region Y)-box 9 protein|campomelic dysplasia, autosomal sex-revers
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Interactome
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Disease
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Publication Reference
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SOX9 is expressed in normal prostate basal cells and regulates androgen receptor expression in prostate cancer cells.
Wang H, McKnight NC, Zhang T, Lu ML, Balk SP, Yuan X.
Cancer Research 2007 Jan; 67(2):528.
Application：IHC-P, WB, Human, C3H10T, CWR22Rv1, RCS cells, Human prostate cancer.
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SOX9 is expressed in normal prostate basal cells and regulates androgen receptor expression in prostate cancer cells.
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