PAK4 (phospho S474) polyclonal antibody
* The price is valid only in USA. Please select country.
-
More Files
- More Functions
Western Blot (Cell lysate)
The cell lysate derived from HEK 293 cells was immunoprobed by PAK4 (phospho S474) polyclonal antibody (Cat # PAB12642) at 1 : 500. An immunoreactive band is observed around ~ 68kDa (Lane 1). This band is blocked by pre-incubation with immunizing peptide (Lane 2).
-
Specification
Product Description
Rabbit polyclonal antibody raised against synthetic phosphopeptide of PAK4.
Immunogen
Synthetic phosphopeptide corresponding to residues surrounding S474 of human PAK4.
Sequence
RKSLV
Host
Rabbit
Theoretical MW (kDa)
68, 90, 80
Reactivity
Human, Mouse, Rat
Specificity
This antibody recognizes the phosphorylated peptide only. It recognizes PAK4 protein at a phosphorylation site of Serine 474(~68 kDa), or serine 602 of PAK5 (~90 kDa) or Serine 560 of PAK6 (~80 kDa). It does not cross react to other PAK1, PAK2 or PAK3.
Form
Liquid
Purification
Phosphospecific-epitope affinity purification
Recommend Usage
ELISA (0.01-0.1 ug/mL)
Immunoprecipitation (2-5 ug/mL)
Western Blot (0.1-1 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (antibody stabilizer).
Storage Instruction
Store at 2-8°C for three month without detectable loss of activity. For long term storage, store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Western Blot (Cell lysate)
The cell lysate derived from HEK 293 cells was immunoprobed by PAK4 (phospho S474) polyclonal antibody (Cat # PAB12642) at 1 : 500. An immunoreactive band is observed around ~ 68kDa (Lane 1). This band is blocked by pre-incubation with immunizing peptide (Lane 2).Immunoprecipitation
Enzyme-linked Immunoabsorbent Assay
-
Gene Info — PAK4
Entrez GeneID
10298Gene Name
PAK4
Gene Alias
-
Gene Description
p21 protein (Cdc42/Rac)-activated kinase 4
Omim ID
605451Gene Ontology
HyperlinkGene Summary
PAK proteins, a family of serine/threonine p21-activating kinases, include PAK1, PAK2, PAK3 and PAK4. PAK proteins are critical effectors that link Rho GTPases to cytoskeleton reorganization and nuclear signaling. They serve as targets for the small GTP binding proteins Cdc42 and Rac and have been implicated in a wide range of biological activities. PAK4 interacts specifically with the GTP-bound form of Cdc42Hs and weakly activates the JNK family of MAP kinases. PAK4 is a mediator of filopodia formation and may play a role in the reorganization of the actin cytoskeleton. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq
Other Designations
p21(CDKN1A)-activated kinase 4|p21-activated kinase 4|protein kinase related to S. cerevisiae STE20, effector for Cdc42Hs
-
Interactome
-
Pathway
-
Disease
-
Publication Reference
-
Requirement for PAK4 in the anchorage-independent growth of human cancer cell lines.
Callow MG, Clairvoyant F, Zhu S, Schryver B, Whyte DB, Bischoff JR, Jallal B, Smeal T.
J Biol Chem 2001 Oct; 277(1):550.
Application：IF, WB, Mouse, NIH/3T3 cells.
-
Requirement for PAK4 in the anchorage-independent growth of human cancer cell lines.
- +1-909-264-1399
+1-909-992-0619
Toll Free : +1-877-853-6098 - +1-909-992-3401
- sales@abnova.com