The whole cell lysates derived from EGF-stimulated A-431 were immunoblotted by MAP2K5 (phospho S311/T315) polyclonal antibody (Cat # PAB12634) at 1 : 1000 (Lane 1). The lane 2 was pre-absorbed by immunizing peptide.
Dot Blot (Peptide)
Dot Blot : 1 ug peptide was blot onto NC membrane. A : MAP2K5 (pS311/pT315). B : MAP2K5 (Non-phospho). C : Non-related phospho-specific. Followed were blotted at a 1 : 1000 dilution by : 1. MAP2K5 (phospho S311/T315) polyclonal antibody (Cat # PAB12634). 2 : MAP2K5 polyclonal antibody (Cat # PAB12705).
The protein encoded by this gene is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase specifically interacts with and activates MAPK7/ERK5. This kinase itself can be phosphorylated and activated by MAP3K3/MEKK3, as well as by atypical protein kinase C isoforms (aPKCs). The signal cascade mediated by this kinase is involved in growth factor stimulated cell proliferation and muscle cell differentiation. Four alternatively spliced transcript variants of this gene encoding distinct isoforms have been described. [provided by RefSeq