MAP2K5 (phospho S311/T315) polyclonal antibody
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More Files
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Western Blot (Cell lysate)
The whole cell lysates derived from EGF-stimulated A-431 were immunoblotted by MAP2K5 (phospho S311/T315) polyclonal antibody (Cat # PAB12634) at 1 : 1000 (Lane 1). The lane 2 was pre-absorbed by immunizing peptide.
Dot Blot (Peptide)
Dot Blot : 1 ug peptide was blot onto NC membrane.
A : MAP2K5 (pS311/pT315).
B : MAP2K5 (Non-phospho).
C : Non-related phospho-specific.
Followed were blotted at a 1 : 1000 dilution by :
1. MAP2K5 (phospho S311/T315) polyclonal antibody (Cat # PAB12634).
2 : MAP2K5 polyclonal antibody (Cat # PAB12705).
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic phosphopeptide of MAP2K5.
Immunogen
Synthetic phosphopeptide corresponding to residues surrounding S311/T315 of human MAP2K5.
Sequence
SIAKT
Host
Rabbit
Theoretical MW (kDa)
49
Reactivity
Human, Mouse, Rat
Specificity
This antibody recognizes ~49 KDa of human MAP2K5 at the phosphorylation site of Ser 311/Thr315. It does not cross react to non-phospho MEK5.
Form
Liquid
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
Western Blot (0.1-1 ug/mL)
ELISA (0.01-0.1 ug/mL)
Immunoprecipitation (2-5 ug/mL)
Immunohistochemistry (2-5 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In TBS, pH 7.2 (BSA, 10% Proclin300)
Storage Instruction
Store at 4°C. For long term storage store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Western Blot (Cell lysate)
The whole cell lysates derived from EGF-stimulated A-431 were immunoblotted by MAP2K5 (phospho S311/T315) polyclonal antibody (Cat # PAB12634) at 1 : 1000 (Lane 1). The lane 2 was pre-absorbed by immunizing peptide.Immunohistochemistry
Immunoprecipitation
Enzyme-linked Immunoabsorbent Assay
Dot Blot (Peptide)
Dot Blot : 1 ug peptide was blot onto NC membrane.
A : MAP2K5 (pS311/pT315).
B : MAP2K5 (Non-phospho).
C : Non-related phospho-specific.
Followed were blotted at a 1 : 1000 dilution by :
1. MAP2K5 (phospho S311/T315) polyclonal antibody (Cat # PAB12634).
2 : MAP2K5 polyclonal antibody (Cat # PAB12705). -
Gene Info — MAP2K5
Entrez GeneID
5607Gene Name
MAP2K5
Gene Alias
HsT17454, MAPKK5, MEK5, PRKMK5
Gene Description
mitogen-activated protein kinase kinase 5
Omim ID
602520Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase specifically interacts with and activates MAPK7/ERK5. This kinase itself can be phosphorylated and activated by MAP3K3/MEKK3, as well as by atypical protein kinase C isoforms (aPKCs). The signal cascade mediated by this kinase is involved in growth factor stimulated cell proliferation and muscle cell differentiation. Four alternatively spliced transcript variants of this gene encoding distinct isoforms have been described. [provided by RefSeq
Other Designations
MAP kinase kinase MEK5b|MAPK/ERK kinase 5|dual specificity mitogen-activated protein kinase kinase 5
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Interactome
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Pathway
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Disease
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Publication Reference
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Effects of MEK5/ERK5 association on small ubiquitin-related modification of ERK5: implications for diabetic ventricular dysfunction after myocardial infarction.
Shishido T, Woo CH, Ding B, McClain C, Molina CA, Yan C, Yang J, Abe J.
Circulation Research 2008 May; 102(11):1416.
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Effects of MEK5/ERK5 association on small ubiquitin-related modification of ERK5: implications for diabetic ventricular dysfunction after myocardial infarction.
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