LRP6 (phospho T1479) polyclonal antibody
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More Files
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Western Blot (Cell lysate)
The cell lysate derived from conditioned NIH/3T3 was immunoprobed by LRP6 (phospho T1479) polyclonal antibody (Cat # PAB12632) at 1 : 500. An immunoreactive band is observed around ~ 180kDa (1). This band is abolished by pre-incubation with immunizing peptide (2).
Dot Blot (Peptide)
Dot Blot : 1 ug peptide was blot onto NC membrane.
A : Non-related phospho-specific peptide.
B : LRP6 (pT1479).
C : Non- Phosphopeptide LRP6 was blotted by LRP6 (phospho T1479) polyclonal antibody (Cat # PAB12632) a 1 : 2000 dilution.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic phosphopeptide of LRP6.
Immunogen
Synthetic phosphopeptide corresponding to residues surrounding T1479 of human LRP6.
Sequence
KGTYF
Host
Rabbit
Theoretical MW (kDa)
180
Reactivity
Human, Mouse, Rat
Specificity
This antibody recognizes ~180 KDa of human LRP6.
Form
Liquid
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
Western Blot (0.1-1 ug/mL)
ELISA (0.01-0.1 ug/mL)
Immunoprecipitation (2-5 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In TBS, pH 7.2 (BSA, 10% Proclin300)
Storage Instruction
Store at 4°C. For long term storage store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Western Blot (Cell lysate)
The cell lysate derived from conditioned NIH/3T3 was immunoprobed by LRP6 (phospho T1479) polyclonal antibody (Cat # PAB12632) at 1 : 500. An immunoreactive band is observed around ~ 180kDa (1). This band is abolished by pre-incubation with immunizing peptide (2).Immunoprecipitation
Enzyme-linked Immunoabsorbent Assay
Dot Blot (Peptide)
Dot Blot : 1 ug peptide was blot onto NC membrane.
A : Non-related phospho-specific peptide.
B : LRP6 (pT1479).
C : Non- Phosphopeptide LRP6 was blotted by LRP6 (phospho T1479) polyclonal antibody (Cat # PAB12632) a 1 : 2000 dilution. -
Gene Info — LRP6
Entrez GeneID
4040Gene Name
LRP6
Gene Alias
ADCAD2, FLJ90062, FLJ90421
Gene Description
low density lipoprotein receptor-related protein 6
Gene Ontology
HyperlinkGene Summary
The LRP6 gene encodes a member of the low density lipoprotein receptor (LDLR) gene family, which consists of cell surface proteins involved in receptor-mediated endocytosis of specific ligands. LDLR proteins are comprised of the same basic structural motifs: an extracellular domain that contains LDLR binding repeats and EGF repeats with associated spacer domains containing the YWTD motif; a single transmembrane-spanning domain; and a C-terminal cytoplasmic domain that generally contains at least 1 copy of the NPXY motif (Brown et al., 1998 [PubMed 9704021]). LRP6 and LRP5 (MIM 603506) function as coreceptors for WNT (MIM 164820) ligands and thus play a role in WNT signaling, which is important in a wide variety of biologic processes during pre- and postnatal life in invertebrates and vertebrates (Kokubu et al., 2004 [PubMed 15469977]).[supplied by OMIM
Other Designations
low density lipoprotein-related protein 6
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Interactome
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Pathway
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Disease
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Publication Reference
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TIM-3 signaling hijacks the canonical Wnt/β-catenin pathway to maintain cancer stemness in acute myeloid leukemia.
Teppei Sakoda, Yoshikane Kikushige, Toshihiro Miyamoto, Hidetoshi Irifune, Takuya Harada, Kiwamu Hatakeyama, Yuya Kunisaki, Koji Kato, Koichi Akashi.
Blood Advances 2023 Feb; bloodadvances.2022008405.
Application：WB-Ce, Human, Human leukemic cells, KASUMI-6, Ki-JK, THP-1 cells.
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Cu/Zn Superoxide Dismutase (Sod1) regulates the canonical Wnt signaling pathway.
Bindu Chandrasekharan, Claudia Montllor-Albalate, Alyson E Colin, Joshua L Andersen, Young C Jang, Amit R Reddi.
Biochemical and Biophysical Research Communications 2021 Jan; 534:720.
Application：WB-Tr, Human, HEK 293 cells.
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SFRPs Are Biphasic Modulators of Wnt-Signaling-Elicited Cancer Stem Cell Properties beyond Extracellular Control.
Liang CJ, Wang ZW, Chang YW, Lee KC, Lin WH, Lee JL.
Cell Reports 2019 Aug; 28(6):1511.
Application：WB, Human, HM20 cells.
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Quantitative analysis of sensitivity to a Wnt3a gradient in determination of the pole-to-pole axis of mitotic cells by using a microfluidic device.
Hiraiwa T, Nakai Y, Yamada TG, Tanimoto R, Kimura H, Matsumoto Y, Miki N, Hiroi N, Funahashi A.
FEBS Open Bio 2018 Nov; 8(12):1920.
Application：IF, Human, SH-SY5Y cells.
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Extracellular LDLR repeats modulate Wnt signaling activity by promoting LRP6 receptor endocytosis mediated by the Itch E3 ubiquitin ligase.
Vijayakumar S, Liu G, Wen HC, Abu Y, Chong R, Nastri H, Bornstein GG, Pan ZQ, Aaronson SA.
Genes & Cancer 2017 Jun; 8(7-8):613.
Application：WB-Ce, Human, HEK 293T cells.
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Astragaloside IV Inhibits the Up-Regulation of Wnt/β-Catenin Signaling in Rats with Unilateral Ureteral Obstruction.
Wang L, Chi YF, Yuan ZT, Zhou WC, Yin PH, Zhang XM, Peng W.
Cellular Physiology and Biochemistry 2014 Apr; 33(5):1316.
Application：WB-Ti, Rat, Kidney.
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Coordinated Action of CK1 Isoforms in Canonical Wnt Signaling.
Del Valle-Perez B, Arques O, Vinyoles M, de Herreros AG, Dunach M.
Molecular and Cellular Biology 2011 Jul; 31(14):2877.
Application：IP-WB, WB-Ce, WB-Tr, Human, SW-480, HEK293 cells.
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A p120-catenin-CK1{varepsilon} complex regulates Wnt signaling.
Casagolda D, del Valle-Perez B, Valls G, Lugilde E, Vinyoles M, Casado-Vela J, Solanas G, Batlle E, Reynolds AB, Casal JI, de Herreros AG, Dunach M.
Journal of Cell Science 2010 Aug; 123(Pt 15):2621.
Application：WB, Human, SW480 cells.
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Analysis of endogenous LRP6 function reveals a novel feedback mechanism by which Wnt negatively regulates its receptor.
Khan Z, Vijayakumar S, de la Torre TV, Rotolo S, Bafico A.
Molecular and Cellular Biology 2007 Oct; 27(20):7291.
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TIM-3 signaling hijacks the canonical Wnt/β-catenin pathway to maintain cancer stemness in acute myeloid leukemia.
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