Western blotting analysis (non - reducing conditions) of mouse Lat2 in mouse spleen tissue. Lane 1 : Immunostaining with Lat2 polyclonal antibody (Cat # PAB1263) . Lane 2 : Immunostaining with Lat2 polyclonal antibody (specific for N - terminal domain of mouse Lat2) .
Distribution of LAT and Lat2 in separate clusters on mast cell plasma membrane sheets. LAT and Lat2 were detected on mouse bone marrow - derived mast cells (BMMC ; A) and RBL rat basophilic leukemia cell line (B) by anti - LAT (LAT - 1D1) and Lat2 polyclonal antibody, followed by 5 or 10 nm gold - conjugated secondary antibodies, respectively.