The cell lysate derived from conditioned NIH/3T3 was immunoprecipitated by IRS2 (phospho S731) polyclonal antibody (Cat # PAB12629), then immunoprobed by the same antibody at 1 : 500 (lane 1). Lane 2 is a negative control.
Dot Blot (Peptide)
Dot Blot : 1 ug peptide was blot onto NC membrane. A : IRS2 (pS731). B : IRS2 (non phosphorylated). C : Non-related Phosphopeptide. Followed by rabbit antibodies incubation at a 1 : 1000 dilution : 1. IRS2 (phospho S731) polyclonal antibody (Cat # PAB12629). 2. IRS2 polyclonal antibody (Cat # PAB12697).
This gene encodes the insulin receptor substrate 2, a cytoplasmic signaling molecule that mediates effects of insulin, insulin-like growth factor 1, and other cytokines by acting as a molecular adaptor between diverse receptor tyrosine kinases and downstream effectors. The product of this gene is phosphorylated by the insulin receptor tyrosine kinase upon receptor stimulation, as well as by an interleukin 4 receptor-associated kinase in response to IL4 treatment. [provided by RefSeq