IRS1 (phospho Y612) polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic phosphopeptide of IRS1.
Immunogen
Synthetic phosphopeptide corresponding to residues surrounding Y612 of human IRS1.
Sequence
DGYMP
Host
Rabbit
Theoretical MW (kDa)
165
Reactivity
Human, Mouse, Rat
Specificity
This antibody recognizes ~165 KDa of human IRS1 (pTyr 612).
Form
Liquid
Purification
Site-modified Epitope Affinity Purification
Isotype
IgG
Recommend Usage
Western Blot (0.1-1 ug/mL)
ELISA (0.01-0.1 ug/mL)
Immunoprecipitation (2-5 ug/mL)
Immunohistochemistry (0.5-2 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In TBS, pH 7.2 (BSA, 10% Proclin300)
Storage Instruction
Store at 4°C. For long term storage store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Western Blot
Immunohistochemistry
Immunoprecipitation
The cell lysate derived from insulin stimulated CHO was immunoprecipitated by IRS1 (phospho Y612) polyclonal antibody (Cat # PAB12628), then immunoprobed by the same antibody at 1 : 500 (Lane 1). Lane 2 is a negative control.Enzyme-linked Immunoabsorbent Assay
Dot Blot (Peptide)
Dot Blot : 1 ug peptide was blot onto NC membrane.
A : IRS1 (pY612).
B : IRS1 (non phosphorylated).
C : Non-related Phosphopeptide.
Followed by rabbit antibodies incubation at a 1 : 1000 dilution :
1. IRS1 (phospho Y612) polyclonal antibody (Cat # PAB12628).
2. IRS1 polyclonal antibody (Cat # PAB12695). -
Gene Info — IRS1
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Interactome
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Pathway
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Disease
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Publication Reference
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High fructose diet induces early mortality via autophagy factors accumulation in the rostral ventrolateral medulla as ameliorated by pioglitazone.
Lin IC, Wu CW, Tain YL, Chen IC, Hung CY, Wu KLH.
The Journal of Nutritional Biochemistry 2019 Jul; 69:87.
Application:WB-Ti, Rat, Rat rostral ventrolateral medulla.
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Tissue and serum IGFBP7 protein as biomarker in high-grade soft tissue sarcoma.
Benassi MS, Pazzaglia L, Novello C, Quattrini I, Pollino S, Magagnoli G, Picci P, Conti A.
American Journal of Cancer Research 2015 Oct; 5(11):3446.
Application:IHC, Human, Soft tissue sarcomas.
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Protein kinase C Theta inhibits insulin signaling by phosphorylating IRS1 at Ser(1101).
Li Y, Soos TJ, Li X, Wu J, Degennaro M, Sun X, Littman DR, Birnbaum MJ, Polakiewicz RD.
The Journal of Biological Chemistry 2004 Sep; 279(44):45304.
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High fructose diet induces early mortality via autophagy factors accumulation in the rostral ventrolateral medulla as ameliorated by pioglitazone.
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