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Last updated: 2017/11/19
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IRS1 (phospho S312) polyclonal antibody

  • Catalog # : PAB12627
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic phosphopeptide of IRS1.
  • Immunogen:
  • Synthetic phosphopeptide corresponding to residues surrounding S312 of human IRS1.
  • Sequence:
  • ATSP
  • Host:
  • Rabbit
  • Theoretical MW (kDa):
  • 165
  • Reactivity:
  • Human, Mouse, Rat
  • Specificity:
  • This antibody recognizes ~165 KDa of human IRS1 (pSer312).
  • Form:
  • Liquid
  • Quality Control Testing:
  • Antibody Reactive Against Synthetic Peptide.
  • Recommend Usage:
  • Western Blot (0.1-1 ug/mL)
    ELISA (0.01-0.1 ug/mL)
    Immunoprecipitation (2-5 ug/mL)
    Immunohistochemistry (0.5-2 ug/mL)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In TBS, pH 7.2 (BSA, 10% Proclin300)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C or lower.
    Aliquot to avoid repeated freezing and thawing.
  • Publication Reference
  • Applications
  • Western Blot
  • Immunohistochemistry
  • Immunoprecipitation
  • Immunoprecipitation
  • The cell lysate derived from insulin stimulated CHO was immunoprecipitated by IRS1 (phospho S312) polyclonal antibody (Cat # PAB12627), then immunoprobed by the same antibody at 1 : 500 (Lane 1). Lane 2 is a negative control.
  • ELISA
  • Dot Blot (Peptide)
  • Dot Blot (Peptide)
  • Dot Blot : 1 ug peptide was blot onto NC membrane.
    A : IRS1 (pS312).
    B : IRS1 (non phosphorylated).
    C : Non-related Phosphopeptide.
    Followed by rabbit antibodies incubation at a 1 : 1000 dilution.
    1. IRS1 (phospho S312) polyclonal antibody (Cat # PAB12627).
    2. IRS1 polyclonal antibody (Cat # PAB12695).
  • Application Image
  • Western Blot
  • Immunohistochemistry
  • ELISA
  • Gene Information
  • Entrez GeneID:
  • 3667
  • Gene Name:
  • IRS1
  • Gene Alias:
  • HIRS-1
  • Gene Description:
  • insulin receptor substrate 1
  • Other Designations:
  • -
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