IRS1 (phospho S312) polyclonal antibody
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More Files
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Immunoprecipitation
The cell lysate derived from insulin stimulated CHO was immunoprecipitated by IRS1 (phospho S312) polyclonal antibody (Cat # PAB12627), then immunoprobed by the same antibody at 1 : 500 (Lane 1). Lane 2 is a negative control.
Dot Blot (Peptide)
Dot Blot : 1 ug peptide was blot onto NC membrane.
A : IRS1 (pS312).
B : IRS1 (non phosphorylated).
C : Non-related Phosphopeptide.
Followed by rabbit antibodies incubation at a 1 : 1000 dilution.
1. IRS1 (phospho S312) polyclonal antibody (Cat # PAB12627).
2. IRS1 polyclonal antibody (Cat # PAB12695).
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic phosphopeptide of IRS1.
Immunogen
Synthetic phosphopeptide corresponding to residues surrounding S312 of human IRS1.
Sequence
ATSPA
Host
Rabbit
Theoretical MW (kDa)
165
Reactivity
Human, Mouse, Rat
Specificity
This antibody recognizes ~165 KDa of human IRS1 (pSer312).
Form
Liquid
Purification
Site-modified epitope affinity purification
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
Western Blot (0.1-1 ug/mL)
ELISA (0.01-0.1 ug/mL)
Immunoprecipitation (2-5 ug/mL)
Immunohistochemistry (0.5-2 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In TBS, pH 7.2 (BSA, 10% Proclin300).
Storage Instruction
Store at 4°C for three months. For long term storage store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Western Blot
Immunohistochemistry
Immunoprecipitation
The cell lysate derived from insulin stimulated CHO was immunoprecipitated by IRS1 (phospho S312) polyclonal antibody (Cat # PAB12627), then immunoprobed by the same antibody at 1 : 500 (Lane 1). Lane 2 is a negative control.Enzyme-linked Immunoabsorbent Assay
Dot Blot (Peptide)
Dot Blot : 1 ug peptide was blot onto NC membrane.
A : IRS1 (pS312).
B : IRS1 (non phosphorylated).
C : Non-related Phosphopeptide.
Followed by rabbit antibodies incubation at a 1 : 1000 dilution.
1. IRS1 (phospho S312) polyclonal antibody (Cat # PAB12627).
2. IRS1 polyclonal antibody (Cat # PAB12695). -
Gene Info — IRS1
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Interactome
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Pathway
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Disease
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Publication Reference
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Protein kinase C Theta inhibits insulin signaling by phosphorylating IRS1 at Ser(1101).
Li Y, Soos TJ, Li X, Wu J, Degennaro M, Sun X, Littman DR, Birnbaum MJ, Polakiewicz RD.
The Journal of Biological Chemistry 2004 Sep; 279(44):45304.
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Protein kinase C Theta inhibits insulin signaling by phosphorylating IRS1 at Ser(1101).
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