NFKBIA (phospho S32/S36) polyclonal antibody
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Western Blot (Cell lysate)
The whole cell lysate derived from TNFa-treated Jurkat cells was immunoblotted by NFKBIA (phospho S32/S36) polyclonal antibody (Cat # PAB12626) at 1 : 500. An immune-reactive band is observed around ~ 42kDa (lane 1). This band is blocked by pre-incubation of immunizing peptide (lane 2).
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic phosphopeptide of NFKBIA.
Immunogen
Synthetic phosphopeptide corresponding to residues surrounding S32/S36 of human NFKBIA.
Sequence
DSGLDSM
Host
Rabbit
Theoretical MW (kDa)
42
Reactivity
Human, Mouse, Rat
Specificity
This antibody recognizes ~42 KDa of human NFKBIA at dual phosphorylation sites of Ser32/Ser36. It does not recognize non-phosphorylated peptides.
Form
Liquid
Recommend Usage
ELISA (0.01-0.1 ug/mL)
Immunoprecipitation (2-5 ug/mL)
Western Blot (0.1-1 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In TBS, pH 7.2 (antibody stabilizer).
Storage Instruction
Store at 4°C for three month. For long term storage, store at -20 to -80°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Western Blot (Cell lysate)
The whole cell lysate derived from TNFa-treated Jurkat cells was immunoblotted by NFKBIA (phospho S32/S36) polyclonal antibody (Cat # PAB12626) at 1 : 500. An immune-reactive band is observed around ~ 42kDa (lane 1). This band is blocked by pre-incubation of immunizing peptide (lane 2).Immunoprecipitation
Enzyme-linked Immunoabsorbent Assay
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Gene Info — NFKBIA
Entrez GeneID
4792Gene Name
NFKBIA
Gene Alias
IKBA, MAD-3, NFKBI
Gene Description
nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha
Omim ID
164008Gene Ontology
HyperlinkGene Summary
NFKB1 (MIM 164011) or NFKB2 (MIM 164012) is bound to REL (MIM 164910), RELA (MIM 164014), or RELB (MIM 604758) to form the NFKB complex. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA or NFKBIB, MIM 604495), which inactivate NF-kappa-B by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA, MIM 600664, or IKBKB, MIM 603258) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime (where H is A, C, or T; R is an A or G purine; and Y is a C or T pyrimidine).[supplied by OMIM
Other Designations
IkappaBalpha|nuclear factor of kappa light chain gene enhancer in B-cells
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Interactome
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Pathway
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Disease
- Adenocarcinoma
- Alcoholism
- Arthritis
- Asthma
- Atherosclerosis
- Behcet Syndrome
- Breast cancer
- Breast Neoplasms
- Bronchiolitis
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Publication Reference
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Inhibition of I kappa B-alpha phosphorylation and degradation and subsequent NF-kappa B activation by glutathione peroxidase overexpression.
Kretz-Remy C, Mehlen P, Mirault ME, Arrigo AP.
The Journal of Cell Biology 1996 Jun; 133(5):1083.
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Inhibition of I kappa B-alpha phosphorylation and degradation and subsequent NF-kappa B activation by glutathione peroxidase overexpression.
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