GSK3A (phospho S21) polyclonal antibody
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More Files
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Western Blot (Cell lysate)
The NIH/3T3 cell lysate derived from conditioned culture medium was resolved onto 10% SDS-PAGE, transferred onto NC membrane, and followed by an immunoblotting with GSK3A (phospho S21) polyclonal antibody (Cat # PAB12623) at 1 : 500 (Lane 1). Lane 2 was pre-incubated with immunizing peptide.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic phosphopeptide of GSK3A.
Immunogen
Synthetic phosphopeptide corresponding to residues surrounding S21 of human GSK3A.
Sequence
RTSSFA
Host
Rabbit
Reactivity
Human, Mouse, Rat
Specificity
This antibody recognizes GSK3A (pS21) with a phosphorylated site at Serine 9. It does not cross-react with non-phosphospecific peptide.
Form
Liquid
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
Western Blot (0.1-1 ug/mL)
ELISA (0.01-0.1 ug/mL)
Immunoprecipitation (2-5 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In TBS, pH 7.2 (BSA, 10% Proclin300)
Storage Instruction
Store at 4°C. For long term storage store at -20°C or lower.
Aliquot to avoid repeated freezing and thawing. -
Applications
Western Blot (Cell lysate)
The NIH/3T3 cell lysate derived from conditioned culture medium was resolved onto 10% SDS-PAGE, transferred onto NC membrane, and followed by an immunoblotting with GSK3A (phospho S21) polyclonal antibody (Cat # PAB12623) at 1 : 500 (Lane 1). Lane 2 was pre-incubated with immunizing peptide.Immunohistochemistry
Immunoprecipitation
Enzyme-linked Immunoabsorbent Assay
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Gene Info — GSK3A
Entrez GeneID
2931Gene Name
GSK3A
Gene Alias
DKFZp686D0638
Gene Description
glycogen synthase kinase 3 alpha
Omim ID
606784Gene Ontology
HyperlinkGene Summary
Glycogen synthase kinase 3-alpha (GSK3A; EC {2.7.1.37}) is a multifunctional protein serine kinase, homologous to Drosophila 'shaggy' (zeste-white3) and implicated in the control of several regulatory proteins including glycogen synthase (see GYS1, {138570}) and transcription factors (e.g., JUN, {165160}). It also plays a role in the WNT ({164820}) and PI3K (see PIK3CG, {601232}) signaling pathways (see review by {1:Ali et al. (2001)}).[supplied by OMIM
Other Designations
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Interactome
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Pathway
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Disease
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Publication Reference
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GSK3alpha exhibits beta-catenin and tau directed kinase activities that are modulated by Wnt.
Asuni AA, Hooper C, Reynolds CH, Lovestone S, Anderton BH, Killick R.
The European Journal of Neuroscience 2006 Dec; 24(12):3387.
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Protein kinase C betaII regulates Akt phosphorylation on Ser-473 in a cell type- and stimulus-specific fashion.
Kawakami Y, Nishimoto H, Kitaura J, Maeda-Yamamoto M, Kato RM, Littman DR, Leitges M, Rawlings DJ, Kawakami T.
The Journal of Biological Chemistry 2004 Nov; 279(46):47720.
Application：WB, Mouse, Mouse bone marrow-derived mast cells.
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GSK3alpha exhibits beta-catenin and tau directed kinase activities that are modulated by Wnt.
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