ESR1 (phospho S167) polyclonal antibody
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More Files
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Western Blot (Cell lysate)
The whole cell lysates derived from MCF-7 were immunoblotted by ESR1 (phopho S167) polyclonal antibody (Cat # PAB12621) at 1 : 500 (Lane 1). The immunoreactive band is blocked by pre-incubation of immunizing peptide (Lane 2).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human breast cancer tissue stained with ESR1 (phospho S167) polyclonal antibody (Cat # PAB12621) at 1 : 200 for 10 min at RT. Staining of formalin-fixed tissue requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic phosphopeptide of ESR1.
Immunogen
Synthetic phosphopeptide corresponding to residues surrounding S167 of human ESR1.
Sequence
LASTN
Host
Rabbit
Theoretical MW (kDa)
67
Reactivity
Human
Specificity
This antibody recognizes ~67 KDa of human ESR1 at the phosphorylation site of SESR1 167. It does not cross react to non-phosphorylated peptide.
Form
Liquid
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
Western Blot (0.1-1 ug/mL)
ELISA (0.01-0.1 ug/mL)
Immunoprecipitation (2-5 ug/mL)
Immunohistochemistry (1:200)
The optimal working dilution should be determined by the end user.Storage Buffer
In TBS, pH 7.2 (BSA, 10% Proclin300)
Storage Instruction
Store at 4°C. For long term storage store at -20°C or lower.
Aliquot to avoid repeated freezing and thawing. -
Applications
Western Blot (Cell lysate)
The whole cell lysates derived from MCF-7 were immunoblotted by ESR1 (phopho S167) polyclonal antibody (Cat # PAB12621) at 1 : 500 (Lane 1). The immunoreactive band is blocked by pre-incubation of immunizing peptide (Lane 2).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human breast cancer tissue stained with ESR1 (phospho S167) polyclonal antibody (Cat # PAB12621) at 1 : 200 for 10 min at RT. Staining of formalin-fixed tissue requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.Immunoprecipitation
Enzyme-linked Immunoabsorbent Assay
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Gene Info — ESR1
Entrez GeneID
2099Gene Name
ESR1
Gene Alias
DKFZp686N23123, ER, ESR, ESRA, Era, NR3A1
Gene Description
estrogen receptor 1
Gene Ontology
HyperlinkGene Summary
This gene encodes an estrogen receptor, a ligand-activated transcription factor composed of several domains important for hormone binding, DNA binding, and activation of transcription. The protein localizes to the nucleus where it may form a homodimer or a heterodimer with estrogen receptor 2. Estrogen and its receptors are essential for sexual development and reproductive function, but also play a role in other tissues such as bone. Estrogen receptors are also involved in pathological processes including breast cancer, endometrial cancer, and osteoporosis. Alternative splicing results in several transcript variants, which differ in their 5' UTRs and use different promoters. [provided by RefSeq
Other Designations
OTTHUMP00000017718|OTTHUMP00000017719|estrogen receptor alpha
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Interactome
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Disease
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Publication Reference
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Ligand-independent interactions of p160/steroid receptor coactivators and CREB-binding protein (CBP) with estrogen receptor-alpha: regulation by phosphorylation sites in the A/B region depends on other receptor domains.
Dutertre M, Smith CL.
Molecular Endocrinology (Baltimore, Md.) 2003 Apr; 17(7):1296.
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Ligand-independent interactions of p160/steroid receptor coactivators and CREB-binding protein (CBP) with estrogen receptor-alpha: regulation by phosphorylation sites in the A/B region depends on other receptor domains.
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