The A-431 cell lysate resolved onto 12% SDS-PAGE, transferred onto NC membrane, and followed by an immunoblotting with CDC2 (phospho Y15) polyclonal antibody (Cat # PAB12614) (Lane 1) at 1 : 500, or with a pre-incubation of immunizing peptide (lane 2).
Dot Blot (Peptide)
Dot Blot : 1 ug peptide was blot onto NC membrane. A : CDC2 (pY15, Phospho-specific). B : CDC2 (Paired Y15) (Non-phospho-speicfic). C : Non-related phospho-specific peptide. Followed were blotted at a 1 : 2000 dilution by : 1. CDC2 (phospho Y15) polyclonal antibody (Cat # PAB12614). 2. CDC2 polyclonal antibody (Cat # PAB12668).
The protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein is a catalytic subunit of the highly conserved protein kinase complex known as M-phase promoting factor (MPF), which is essential for G1/S and G2/M phase transitions of eukaryotic cell cycle. Mitotic cyclins stably associate with this protein and function as regulatory subunits. The kinase activity of this protein is controlled by cyclin accumulation and destruction through the cell cycle. The phosphorylation and dephosphorylation of this protein also play important regulatory roles in cell cycle control. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
OTTHUMP00000019660,cell cycle controller CDC2,cell division control protein 2 homolog,cell division cycle 2 protein,cyclin-dependent kinase 1,p34 protein kinase