The cell lysate derived from HeLa was immunoprobed at a dilution of 1 : 500 by the following antibodies. 1 : CTNNB1 (phospho Y228) polyclonal antibody (Cat # PAB12605). 2 : CTNNB1 polyclonal antibody (Cat # PAB12659). 3 : CTNNB1 (phospho Y280) polyclonal antibody (Cat # PAB12606). 4: CTNNB1 polyclonal antibody (Cat # PAB12660). 5 : Negative control.
Dot Blot (Peptide)
Dot Blot : 1 ug peptide was blot onto NC membrane. A : Phospho-CTNNB1 Y228. B : CTNNB1 (Non-Phospho). C: Non-related Phosphopeptide Followed by immunoblotting CTNNB1 (phospho Y228) polyclonal antibody (Cat # PAB12605) at 1 : 1000.
Beta-catenin is an adherens junction protein. Adherens junctions (AJs; also called the zonula adherens) are critical for the establishment and maintenance of epithelial layers, such as those lining organ surfaces. AJs mediate adhesion between cells, communicate a signal that neighboring cells are present, and anchor the actin cytoskeleton. In serving these roles, AJs regulate normal cell growth and behavior. At several stages of embryogenesis, wound healing, and tumor cell metastasis, cells form and leave epithelia. This process, which involves the disruption and reestablishment of epithelial cell-cell contacts, may be regulated by the disassembly and assembly of AJs. AJs may also function in the transmission of the 'contact inhibition' signal, which instructs cells to stop dividing once an epithelial sheet is complete.[supplied by OMIM