Scarb1 polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against recombinant Scarb1.
Immunogen
Recombinant protein corresponding to Scarb1.
Host
Rabbit
Reactivity
Human, Mouse
Specificity
This antibody is specific to SR-BI. It can block human SR-BI/Cla-1 (tested in HepG2 cells).
Form
Liquid
Quality Control Testing
Antibody Reactive Against Scarb1.
Recommend Usage
Immunoprecipitation (1:100)
Blocking (1:500-1000)
Immunocytochemistry (1:50-1:200)
Immunofluorescence (1:50-1:200)
The optimal working dilution should be determined by the end user.Storage Buffer
In antiserum (0.05% sodium azide)
Storage Instruction
Store at -20°C or -80°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
Western blot analysis of Scarb1 (80kDa) from mouse testis lysate total protein using Scarb1 polyclonal antibody (Cat #PAB12597).Immunocytochemistry
Immunofluorescence
Immunoprecipitation
Blocking
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Gene Info — Scarb1
Entrez GeneID
20778Gene Name
Scarb1
Gene Alias
AI120173, CD36, Cd36l1, Cla-1, Cla1, D5Ertd460e, SR-B1, SR-BI, SRBI, Srb1, mSR-BI
Gene Description
scavenger receptor class B, member 1
Gene Ontology
HyperlinkGene Summary
O
Other Designations
OTTMUSP00000026063|scavenger receptor class B type I|scavenger receptor class B1
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Publication Reference
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Increased cholesterol deposition, expression of scavenger receptors, and response to chemotactic factors in Abca1-deficient macrophages.
Francone OL, Royer L, Boucher G, Haghpassand M, Freeman A, Brees D, Aiello RJ.
Arteriosclerosis, Thrombosis, and Vascular Biology 2005 Jun; 25(6):1198.
Application:Flow Cyt, Mouse, Peritoneal exudate cells.
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Different transport routes for high density lipoprotein and its associated free sterol in polarized hepatic cells.
Wustner D, Mondal M, Huang A, Maxfield FR.
Journal of Lipid Research 2004 Mar; 45(3):427.
Application:Func, IF, Human, HepG2 cells.
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Scavenger receptor class B, type I-mediated [3H]cholesterol efflux to high and low density lipoproteins is dependent on lipoprotein binding to the receptor.
Gu X, Kozarsky K, Krieger M.
The Journal of Biological Chemistry 2000 Sep; 275(39):29993.
Application:Func, IF, WB-Tr, Human, Monkey, COS M6, ldlA-7 cells.
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Increased cholesterol deposition, expression of scavenger receptors, and response to chemotactic factors in Abca1-deficient macrophages.
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