YAP1 polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against recombinant YAP1.
Immunogen
Recombinant protein corresponding to YAP1.
Host
Rabbit
Reactivity
Human
Form
Liquid
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Recommend Usage
Immunocytochemistry (1:50-1:200)
Immunofluorescence (1:50-1:200)
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (10% glycerol, 0.02% sodium azide)
Storage Instruction
Store at -20°C or -80°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western blot analysis of YAP1 using YAP1 polyclonal antibody (Cat #PAB12570) in transfected HEK 293 cell lysate.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunocytochemistry
Immunofluorescence
Immunoprecipitation
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Publication Reference
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Transforming properties of YAP, a candidate oncogene on the chromosome 11q22 amplicon.
Overholtzer M, Zhang J, Smolen GA, Muir B, Li W, Sgroi DC, Deng CX, Brugge JS, Haber DA.
PNAS 2006 Aug; 103(33):12405.
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Cross-species oncogenomics in cancer gene identification.
Peeper D, Berns A.
Cell 2006 Jun; 125(7):1230.
Application:IHC-P, WB-Ti, Human, Mouse, Human and murine amplicon-containing tumors.
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Molecular profiling of transformed and metastatic murine squamous carcinoma cells by differential display and cDNA microarray reveals altered expression of multiple genes related to growth, apoptosis, angiogenesis, and the NF-kappaB signal pathway.
Dong G, Loukinova E, Chen Z, Gangi L, Chanturita TI, Liu ET, Van Waes C.
Cancer Research 2001 Jun; 31(12):4797.
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Transforming properties of YAP, a candidate oncogene on the chromosome 11q22 amplicon.
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