Rabbit polyclonal antibody raised against full length recombinant MARCKS.
Recombinant protein corresponding to full length MARCKS.
The MARCKS protein runs at about 80KDa on SDS-PAGE gels, although the real molecular weight is much lower, at about 31.5KDa. The reason for this is probably because the protein is extremely rich in acidic residues. Such proteins appear to bind less SDS than the average protein, and so migrate in PAGE more slowly than the normal protein.
Quality Control Testing:
Antibody Reactive Against Recombinant Protein.
Immunocytochemistry (1:5000) Immunofluorescence (1:500-1:1000) Western Blot (1:20000) The optimal working dilution should be determined by the end user.
Store at 4°C for short term. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing.
Western blot analysis of MARCKS in whole rat cortex (Co), cerebellum (Ce), brain stem (BS) and spinal cord (SC) homogenate stained with MARCKS polyclonal antibody (Cat # PAB12564) at dilution of 1 : 10,000.
View of mixed neuron/glial cultures stained with our MARCKS antibody (red) and MARCKS polyclonal antibody (Cat # PAB12564), our antibody to MAP2 made in chicken. Note that the MARCKS antibody stains vesicular structures both in the glial cells and in the dendrites of the neurons, which are strongly stained with the MAP2 antibody.