MAP1LC3B polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of MAP1LC3B in treated U87 MG (human glioblastoma astrocytoma) lysates using MAP1LC3B polyclonal antibody (Cat # PAB12534).
Immunohistochemistry
LC3B staining of MAP1LC3B in gliobastoma multiform tissue using MAP1LC3B polyclonal antibody (Cat # PAB12534).
Immunohistochemistry
LC3B staining of MAP1LC3B in treated U87-MG cultured & subcutaneous tumors using MAP1LC3B polyclonal antibody (Cat # PAB12534).
Immunohistochemistry
Staining of MAP1LC3B on treated U373-MG (human glioblastoma) cells using MAP1LC3B polyclonal antibody (Cat # PAB12534).
Immunofluorescence
Immunofluorescent staining of MAP1LC3B on treated U373-MG cells using MAP1LC3B polyclonal antibody (Cat # PAB12534). The nuclei were stained with DAPI.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of MAP1LC3B.
Immunogen
A synthetic peptide corresponding to amino acids 1-50 at N-terminus of human MAP1LC3B.
Host
Rabbit
Reactivity
Human, Mouse
Specificity
This antibody is specific to the B isoform of LC3.
Form
Liquid
Recommend Usage
Flow Cytometry (1:200)
Immunocytochemistry (1:2000)
Immunofluorescence (1:2000)
Immunohistochemistry (Frozen sections) (1:2000)
Western Blot (1:3000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.02% sodium azide)
Storage Instruction
Store at -20°C or -80°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of MAP1LC3B in treated U87 MG (human glioblastoma astrocytoma) lysates using MAP1LC3B polyclonal antibody (Cat # PAB12534).Immunohistochemistry (Frozen sections)
Immunohistochemistry
LC3B staining of MAP1LC3B in gliobastoma multiform tissue using MAP1LC3B polyclonal antibody (Cat # PAB12534).Immunohistochemistry
LC3B staining of MAP1LC3B in treated U87-MG cultured & subcutaneous tumors using MAP1LC3B polyclonal antibody (Cat # PAB12534).Immunohistochemistry
Staining of MAP1LC3B on treated U373-MG (human glioblastoma) cells using MAP1LC3B polyclonal antibody (Cat # PAB12534).Immunocytochemistry
Immunofluorescence
Immunofluorescent staining of MAP1LC3B on treated U373-MG cells using MAP1LC3B polyclonal antibody (Cat # PAB12534). The nuclei were stained with DAPI.Flow Cytometry
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Gene Info — MAP1LC3B
Entrez GeneID
81631Gene Name
MAP1LC3B
Gene Alias
LC3B, MAP1A/1BLC3
Gene Description
microtubule-associated protein 1 light chain 3 beta
Gene Ontology
HyperlinkGene Summary
The product of this gene is a subunit of neuronal microtubule-associated MAP1A and MAP1B proteins, which are involved in microtubule assembly and important for neurogenesis. Studies on the rat homolog implicate a role for this gene in autophagy, a process that involves the bulk degradation of cytoplasmic component. [provided by RefSeq
Other Designations
microtubule-associated proteins 1A/1B light chain 3
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Interactome
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Publication Reference
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The myoblast C2C12 transfected with mutant valosin-containing protein exhibits delayed stress granule resolution on oxidative stress.
Rodriguez-Ortiz CJ, Flores JC, Valenzuela JA, Rodriguez GJ, Zumkehr J, Tran DN, Kimonis VE, Kitazawa M.
The American Journal of Pathology 2016 Apr; 186(6):1623.
Application：WB, IF, Mouse, C2C12 mouse myoblasts.
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Induction of autophagic cell death by a novel molecule is increased by hypoxia.
Tafani M, Schito L, Anwar T, Indelicato M, Sale P, Di Vito M, Morgante E, Beraldi R, Makovec F, Letari O, Caselli G, Spadafora C, Pucci B, Russo MA.
Autophagy 2008 Nov; 4(8):1042.
Application：IF, IP, WB, Human , MDA-MB-231 cells.
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OSU-03012, a novel celecoxib derivative, induces reactive oxygen species-related autophagy in hepatocellular carcinoma.
Gao M, Yeh PY, Lu YS, Hsu CH, Chen KF, Lee WC, Feng WC, Chen CS, Kuo ML, Cheng AL.
Cancer Research 2008 Nov; 68(22):9348.
Application：IF, WB, Human, Huh7 cells.
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Compensatory activation of ERK1/2 in Atg5-deficient mouse embryo fibroblasts suppresses oxidative stress-induced cell death.
Pyo JO, Nah J, Kim HJ, Lee HJ, Heo J, Lee H, Jung YK.
Autophagy 2008 Apr; 4(3):315.
Application：WB-Ce, Mouse, MEF cells.
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Evidence that curcumin suppresses the growth of malignant gliomas in vitro and in vivo through induction of autophagy: role of Akt and extracellular signal-regulated kinase signaling pathways.
Aoki H, Takada Y, Kondo S, Sawaya R, Aggarwal BB, Kondo Y.
Molecular Pharmacology 2007 Jul; 72(1):29.
Application：WB, Human, U373-MG, U87-MG cells.
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The myoblast C2C12 transfected with mutant valosin-containing protein exhibits delayed stress granule resolution on oxidative stress.
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