FANCD2 (phospho S222) polyclonal antibody
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More Files
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Western Blot (Cell lysate)
Indicated lymphoblasts (PD7, WT : GM1526, AT) were irradiated with 15 Gy (2), and immunoblotted with anti-FANCD2 and FANCD2 (phospho S222) polyclonal antibody (Cat # PAB12530).
Western Blot (Transfected lysate)
FA-D2 fibroblasts were stably transfected with either pMMP (empty vector, A), FANCD2 (wt, B), FANCD2 (S222A, C), FANCD2 (triple mutant, D), or FANCD2 (quadruple mutant, E). Cells were irradiated with 15 Gy (+), and whole cell lysates were immunoblotted with either anti-FANCD2 or Phospho-FANCD2 S222 polyclonal antibody, (Cat # PAB12530).
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic phosphopeptide of FANCD2.
Immunogen
Synthetic phosphopeptide corresponding to residues surrounding S222 of human FANCD2.
Host
Rabbit
Reactivity
Human
Specificity
This antibody is specific to the phosphorylated form of FANCD2.
Form
Liquid
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
The optimal working dilution should be determined by the end user.
Storage Buffer
No additive
Storage Instruction
Store at -20°C or -80°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Western Blot (Cell lysate)
Indicated lymphoblasts (PD7, WT : GM1526, AT) were irradiated with 15 Gy (2), and immunoblotted with anti-FANCD2 and FANCD2 (phospho S222) polyclonal antibody (Cat # PAB12530).Western Blot (Transfected lysate)
FA-D2 fibroblasts were stably transfected with either pMMP (empty vector, A), FANCD2 (wt, B), FANCD2 (S222A, C), FANCD2 (triple mutant, D), or FANCD2 (quadruple mutant, E). Cells were irradiated with 15 Gy (+), and whole cell lysates were immunoblotted with either anti-FANCD2 or Phospho-FANCD2 S222 polyclonal antibody, (Cat # PAB12530). -
Gene Info — FANCD2
Entrez GeneID
2177Gene Name
FANCD2
Gene Alias
DKFZp762A223, FA-D2, FA4, FACD, FAD, FAD2, FANCD, FLJ23826
Gene Description
Fanconi anemia, complementation group D2
Omim ID
227646Gene Ontology
HyperlinkGene Summary
The Fanconi anemia complementation group (FANC) currently includes FANCA, FANCB, FANCC, FANCD1 (also called BRCA2), FANCD2, FANCE, FANCF, FANCG, FANCI, FANCJ (also called BRIP1), FANCL, FANCM and FANCN (also called PALB2). The previously defined group FANCH is the same as FANCA. Fanconi anemia is a genetically heterogeneous recessive disorder characterized by cytogenetic instability, hypersensitivity to DNA crosslinking agents, increased chromosomal breakage, and defective DNA repair. The members of the Fanconi anemia complementation group do not share sequence similarity; they are related by their assembly into a common nuclear protein complex. This gene encodes the protein for complementation group D2. This protein is monoubiquinated in response to DNA damage, resulting in its localization to nuclear foci with other proteins (BRCA1 AND BRCA2) involved in homology-directed DNA repair. Alternative splicing results in two transcript variants encoding different isoforms. [provided by RefSeq
Other Designations
Fanconi anemia complementation group D2|type 4 Fanconi pancytopenia
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Interactome
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Disease
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Publication Reference
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Convergence of the fanconi anemia and ataxia telangiectasia signaling pathways.
Taniguchi T, Garcia-Higuera I, Xu B, Andreassen PR, Gregory RC, Kim ST, Lane WS, Kastan MB, D'Andrea AD.
Cell 2002 May; 109(4):459.
Application：WB-Tr, Human, FA-D2 fibroblasts.
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Convergence of the fanconi anemia and ataxia telangiectasia signaling pathways.
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