BHLHE40 polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against partial recombinant BHLHE40.
Immunogen
Recombinant protein corresponding to C-terminus of human BHLHE40.
Host
Rabbit
Reactivity
Human
Form
Liquid
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Recommend Usage
The optimal working dilution should be determined by the end user.
Storage Buffer
In Tris-citrate/phosphate buffer, pH 7.0-8.0 (0.09% sodium azide)
Storage Instruction
Store at 4°C. Do not freeze.
Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of BHLHE40 in whole cell lysate from HeLa (50 ug) using BHLHE40 polyclonal antibody (Cat # PAB12512) at 0.1 ug /mL .Immunoprecipitation
Whole cell lysate from 293T (1 mg/IP, 20% of IP loaded) cells. Antibodies : BHLHE40 polyclonal antibody (Cat # PAB12512) used for WB at 1 ug /mL and for IP at 3 ug /mg lysate. DEC1 was also immunoprecipitated using 2 other rabbit anti-DEC1 antibodies, which recognize upstream epitopes. Lanes 1 and 2 : other anti-DEC1 antibodies. Lane 3 : BHLHE40 polyclonal antibody (Cat # PAB12512). Lane 4 : Control IgG IP. -
Gene Info — BHLHE40
Entrez GeneID
8553Protein Accession#
NP_003661.1Gene Name
BHLHE40
Gene Alias
BHLHB2, DEC1, FLJ99214, SHARP-2, STRA13, Stra14
Gene Description
basic helix-loop-helix family, member e40
Omim ID
604256Gene Ontology
HyperlinkGene Summary
This gene encodes a basic helix-loop-helix protein expressed in various tissues. Expression in the chondrocytes is responsive to the addition of Bt2cAMP. The encoded protein is believed to be involved in the control of cell differentiation. [provided by RefSeq
Other Designations
basic helix-loop-helix domain containing, class B, 2|differentially expressed in chondrocytes 1|differentiated embryo chondrocyte expressed gene 1
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Interactome
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Pathway
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Disease
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Publication Reference
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Phospho-ΔNp63α/microRNA feedback regulation in squamous carcinoma cells upon cisplatin exposure.
Huang Y, Kesselman D, Kizub D, Guerrero-Preston R, Ratovitski EA.
Cell Cycle 2013 Feb; 12(4):684.
Application:WB, Human, SCC-11 cells.
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Phospho-ΔNp63α/microRNA feedback regulation in squamous carcinoma cells upon cisplatin exposure.
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