ACTG1 polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of ACTG1 in NIH/3T3 (20 ug) lysates using ACTG1 polyclonal antibody (Cat # PAB12510). Lane 1 - 1 : 5,000 dilution. Lane 2 - 1 : 10,000 dilution. Lane 3 - 1 : 15,000 dilution.
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Specification
Product Description
Rabbit polyclonal antibody raised against partial recombinant ACTG1.
Immunogen
Recombinant protein corresponding to N-terminus of human ACTG1.
Sequence
N-terminus
Host
Rabbit
Reactivity
Human, Mouse
Specificity
Detects a single clean band at 40kD representing actin.
Form
Liquid
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Recommend Usage
Immunocytochemistry (1:50-1:200)
Immunofluorescence (1:50-1:200)
Immunoprecipitation (2-5 ug/mL)
Western Blot (1:500)
The optimal working dilution should be determined by the end user.Storage Buffer
In TBS (0.1% BSA, 0.09% sodium azide)
Storage Instruction
Store at 4°C. Do not freeze.
Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of ACTG1 in NIH/3T3 (20 ug) lysates using ACTG1 polyclonal antibody (Cat # PAB12510). Lane 1 - 1 : 5,000 dilution. Lane 2 - 1 : 10,000 dilution. Lane 3 - 1 : 15,000 dilution.Immunocytochemistry
Immunofluorescence
Immunoprecipitation
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Gene Info — ACTG1
Entrez GeneID
71Protein Accession#
P63261Gene Name
ACTG1
Gene Alias
ACT, ACTG, DFNA20, DFNA26
Gene Description
actin, gamma 1
Gene Ontology
HyperlinkGene Summary
Actins are highly conserved proteins that are involved in various types of cell motility, and maintenance of the cytoskeleton. In vertebrates, three main groups of actin isoforms, alpha, beta and gamma have been identified. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins co-exist in most cell types as components of the cytoskeleton, and as mediators of internal cell motility. Actin, gamma 1, encoded by this gene, is a cytoplasmic actin found in nonmuscle cells. [provided by RefSeq
Other Designations
actin, cytoplasmic 2|actin, gamma 1 propeptide|cytoskeletal gamma-actin
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Interactome
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Pathway
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Publication Reference
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Genome-wide discovery of loci influencing chemotherapy cytotoxicity.
Watters JW, Kraja A, Meucci MA, Province MA, McLeod HL.
PNAS 2004 Aug; 101(32):11809.
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Genome-wide discovery of loci influencing chemotherapy cytotoxicity.
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