UCHL1 polyclonal antibody
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More Files
- More Functions
Western Blot (Tissue lysate)
Western blot analysis of whole bovine brain extract stained with UCHL1 polyclonal antibody (Cat # PAB12509) showing a strong and clean band at about 24kDa.
Immunofluorescence
Shows rat mixed neuron/glial cultures stained with UCHL1 polyclonal antibody (Cat # PAB12509) (green). Blue is a DNA stain. Note that the UCHL1 stains neurons strongly and specifically, and that the staining is concentrated in the cell bodies, though some does extend into the dendrites also.
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Specification
Product Description
Rabbit polyclonal antibody raised against full length recombinant UCHL1.
Immunogen
Recombinant protein corresponding to full length human UCHL1.
Host
Rabbit
Reactivity
Human
Form
Liquid
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Recommend Usage
Immunofluorescence (1:500-1:1000)
Western Blot (1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In antiserum (10 mM sodium azide)
Storage Instruction
Store at 4°C for short term. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
Western blot analysis of whole bovine brain extract stained with UCHL1 polyclonal antibody (Cat # PAB12509) showing a strong and clean band at about 24kDa.Immunohistochemistry
Immunofluorescence
Shows rat mixed neuron/glial cultures stained with UCHL1 polyclonal antibody (Cat # PAB12509) (green). Blue is a DNA stain. Note that the UCHL1 stains neurons strongly and specifically, and that the staining is concentrated in the cell bodies, though some does extend into the dendrites also. -
Gene Info — UCHL1
Entrez GeneID
7345Gene Name
UCHL1
Gene Alias
PARK5, PGP9.5, Uch-L1
Gene Description
ubiquitin carboxyl-terminal esterase L1 (ubiquitin thiolesterase)
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene belongs to the peptidase C12 family. This enzyme is a thiol protease that hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. This gene is specifically expressed in the neurons and in cells of the diffuse neuroendocrine system. Mutations in this gene may be associated with Parkinson disease
Other Designations
ubiquitin C-terminal esterase L1|ubiquitin carboxyl-terminal esterase L1|ubiquitin thiolesterase L1
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Interactome
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Disease
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Publication Reference
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Activity Based Profiling of Deubiquitylating Enzymes and Inhibitors in Animal Tissues.
McLellan L, Forder C, Cranston A, Harrigan J, Jacq X.
Methods in Molecular Biology (Clifton, N.J.) 2016 Sep; 1449:411.
Application：WB-Ti, Mouse, Mouse brain, kindey, lung, testis, ovary.
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The UCH-L1 gene encodes two opposing enzymatic activities that affect alpha-synuclein degradation and Parkinson's disease susceptibility.
Liu Y, Fallon L, Lashuel HA, Liu Z, Lansbury PT Jr.
Cell 2002 Oct; 111(2):209.
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Loss of Uch-L1 and Uch-L3 leads to neurodegeneration, posterior paralysis and dysphagia.
Kurihara LJ, Kikuchi T, Wada K, Tilghman SM.
Human Molecular Genetics 2001 Sep; 10(18):1963.
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The neuron-specific protein PGP 9.5 is a ubiquitin carboxyl-terminal hydrolase.
K D Wilkinson, K M Lee, S Deshpande, P Duerksen-Hughes, J M Boss, J Pohl.
Science 1989 Nov; 46(4930):670.
Application：AFC, WB-Re, WB-Ti, Bovine, Bovine thymus, Recombinant proteins.
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Activity Based Profiling of Deubiquitylating Enzymes and Inhibitors in Animal Tissues.
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