Western blot analysis of AANAT using AANAT polyclonal antibody (Cat # PAB12505). Samples of 1E7 homogenates and bacterially expressed hAANAT were loaded, which contained similar levels of AANAT activity.
AANAT in COS-7 and 1E7 cells detected by ICC using AANAT polyclonal antibody (PAB12505). Cells were grown on two-well chamber slides and fixed with 2.5% glutaraldehyde; 1E7 cells treated with FSK for 3 h (two magnifications). COS, COS-7 cells treated with FSK for 3 h.
Arylalkylamine N-acetyltransferase belongs to the superfamily of acetyltransferases. It is the penultimate enzyme in melatonin synthesis and controls the night/day rhythm in melatonin production in the vertebrate pineal gland. Melatonin is essential for seasonal reproduction, modulates the function of the circadian clock in the suprachiasmatic nucleus, and influences activity and sleep. This enzyme is rapidly inactivated when animals are exposed to light at night. This protein is 80% identical to sheep and rat AA-NAT. Arylalkylamine N-acetyltransferase may contribute a multifactorial genetic diseases such as altered behavior in sleep/wake cycle. [provided by RefSeq