AANAT polyclonal antibody
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More Files
- More Functions
Western Blot
Western blot analysis of AANAT using AANAT polyclonal antibody (Cat # PAB12505). Samples of 1E7 homogenates and bacterially expressed hAANAT were loaded, which contained similar levels of AANAT activity.
Immunocytochemistry
AANAT in COS-7 and 1E7 cells detected by ICC using AANAT polyclonal antibody (PAB12505). Cells were grown on two-well chamber slides and fixed with 2.5% glutaraldehyde; 1E7 cells treated with FSK for 3 h (two magnifications). COS, COS-7 cells treated with FSK for 3 h.
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Specification
Product Description
Rabbit polyclonal antibody raised against AANAT.
Immunogen
Human AANAT.
Host
Rabbit
Reactivity
Human, Primates
Specificity
This antibody is specific to primate AANAT.
Form
Liquid
Quality Control Testing
Antibody Reactive Against AANAT.
Recommend Usage
Western Blot (1:500-1:1000)
Immunocytochemistry (1:200)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.1% BSA, 0.05% thimerosal)
Storage Instruction
Store at 4°C for short term. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains thimerosal: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of AANAT using AANAT polyclonal antibody (Cat # PAB12505). Samples of 1E7 homogenates and bacterially expressed hAANAT were loaded, which contained similar levels of AANAT activity.Immunocytochemistry
AANAT in COS-7 and 1E7 cells detected by ICC using AANAT polyclonal antibody (PAB12505). Cells were grown on two-well chamber slides and fixed with 2.5% glutaraldehyde; 1E7 cells treated with FSK for 3 h (two magnifications). COS, COS-7 cells treated with FSK for 3 h. -
Gene Info — AANAT
Entrez GeneID
15Protein Accession#
U40347Gene Name
AANAT
Gene Alias
AA-NAT, SNAT
Gene Description
arylalkylamine N-acetyltransferase
Omim ID
600950Gene Ontology
HyperlinkGene Summary
Arylalkylamine N-acetyltransferase belongs to the superfamily of acetyltransferases. It is the penultimate enzyme in melatonin synthesis and controls the night/day rhythm in melatonin production in the vertebrate pineal gland. Melatonin is essential for seasonal reproduction, modulates the function of the circadian clock in the suprachiasmatic nucleus, and influences activity and sleep. This enzyme is rapidly inactivated when animals are exposed to light at night. This protein is 80% identical to sheep and rat AA-NAT. Arylalkylamine N-acetyltransferase may contribute a multifactorial genetic diseases such as altered behavior in sleep/wake cycle. [provided by RefSeq
Other Designations
serotonin N-acetyltransferase
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Interactome
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Pathway
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Disease
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Publication Reference
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Arylalkylamine N-acetyltransferase: "the Timezyme".
Klein DC.
The Journal of Biological Chemistry 2007 Feb; 282(7):4233.
Application：IHC, Fish, Pinealocyte, Retinal photoreceptor.
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Melatonin synthesis enzymes in Macaca mulatta: focus on arylalkylamine N-acetyltransferase (EC 2.3.1.87).
Coon SL, Del Olmo E, Young WS 3rd, Klein DC.
The Journal of Clinical Endocrinology and Metabolism 2002 Oct; 87(10):4699.
Application：WB-Ti, Monkey, Pineal glands, Retinas.
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14-3-3 Proteins and photoneuroendocrine transduction: role in controlling the daily rhythm in melatonin.
Klein DC, Ganguly S, Coon S, Weller JL, Obsil T, Hickman A, Dyda F.
Biochemical Society Transactions 2002 Aug; 30(4):365.
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Retinal melatonin production: role of proteasomal proteolysis in circadian and photic control of arylalkylamine N-acetyltransferase.
Iuvone PM, Brown AD, Haque R, Weller J, Zawilska JB, Chaurasia SS, Ma M, Klein DC.
Investigative Ophthalmology & Visual Science 2002 Feb; 43(2):564.
Application：WB-Ti, Chicken, Retina, Pineal gland.
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cAmp regulation of arylalkylamine N-acetyltransferase (AANAT, EC 2.3.1.87): a new cell line (1E7) provides evidence of intracellular AANAT activation.
Coon SL, Weller JL, Korf HW, Namboodiri MA, Rollag M, Klein DC.
The Journal of Biological Chemistry 2001 Jun; 276(26):24097.
Application：ICC, WB-Ce, Monkey, COS-7, 1E7 cells.
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Arylalkylamine N-acetyltransferase: "the Timezyme".
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