Western blot analysis of ATRIP in whole cell lysate (100 ug for WB) from HeLa (lanes 1 and 3) and 293-T cells (lanes 2 and 4) using ATRIP polyclonal antibody (Cat # PAB12476). Antibody concentration : 0.2 ug /mL for Lane 1 and 2, 0.1 ug /mL for Lane 3 and 4.
Whole cell lysate (400 ug for IP) from HeLa cells. Antibody used : Lane 1 : ATRIP polyclonal antibody (Cat # PAB12476), Lane 2 : Control Ab, Lane 3 : Normal IgG.
The product of this gene is an essential component of the DNA damage checkpoint, and binds to single-stranded DNA coated with replication protein A that accumulates at sites of DNA damage. The encoded protein interacts with the ataxia telangiectasia and Rad3 related protein, a checkpoint kinase, resulting in accumulation of the kinase at intranuclear foci induced by DNA damage. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq