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Last updated: 2020/8/9
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CIC polyclonal antibody 

  • Catalog # : PAB12458
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against partial recombinant CIC.
  • Immunogen:
  • Recombinant protein corresponding to a region within residue 1500-1608 of human CIC.
  • Host:
  • Rabbit
  • Theoretical MW (kDa):
  • 250
  • Reactivity:
  • Dog, Human, Mouse, Rat
  • Form:
  • Liquid
  • Purification:
  • Immunogen affinity purification
  • Recommend Usage:
  • Immunocytochemistry (1:50)
    Immunofluorescence (1:50)
    Immunoprecipitation (2-5 ug/mg lysate)
    Western Blot (1:1000-1:2500)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS (0.02% sodium azide).
  • Storage Instruction:
  • Store at 4°C for short term. For long term storage, aliquot and store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot
  • Western Blot
  • Western blot analysis of human Capicua /CIC in whole cell lysates of HeLa (5, 15 and 50 ug; Lane 1, Lane 2 and Lane 3) and 293T (T, 50 ug; Lane 4) cells using CIC polyclonal antibody (Cat # PAB12458) used at 1 ug/mL concentration. In Western blot, a band is observed at ~250 kDa. CIC (~164 kDa) interacts with Ataxin 1 (~87 kDa) which may explain the band observed at 250 kDa (complex).
  • Immunocytochemistry
  • Immunofluorescence
  • Immunoprecipitation
  • Immunoprecipitation
  • Detection of Human Capicua/CIC in 1 mg of whole cell lysate from HeLa cells (20% of IP loaded) using CIC polyclonal antibody (Cat # PAB12458) at a concentration of 3 ug/mg lysate. Capicua/CIC was immunoprecipitated by CIC polyclonal antibody (Cat # PAB12457) also, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes.
  • Application Image
  • Immunocytochemistry
  • Immunofluorescence
  • Gene Information
  • Protein Accession#:
  • NP_055940.3; Q96RK0
  • Gene Name:
  • CIC
  • Gene Alias:
  • KIAA0306
  • Gene Description:
  • capicua homolog (Drosophila)
  • Other Designations:
  • capicua homolog
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