CIC polyclonal antibody
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More Files
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Immunofluorescence
Immunofluorescent staining of CIC polyclonal antibody (Cat # PAB12457) was tested in Neuro 2A cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).
Immunoprecipitation
Detection of Human CIC/Capicua by Western blot of Immunoprecipitates. Whole cell lysate from HeLa cells. CIC polyclonal antibody (Cat # PAB12457) used for IP at 3 mcg/mg lysate. CIC was also IPed by rabbit anti-CICa antibody.
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Specification
Product Description
Rabbit polyclonal antibody raised against partial recombinant CIC.
Immunogen
Recombinant protein corresponding to a region within residue 1150-1200 of human CIC.
Host
Rabbit
Theoretical MW (kDa)
164
Reactivity
Dog, Human, Mouse, Rat
Form
Liquid
Purification
Immunogen affinity purification
Recommend Usage
Immunocytochemistry (1:500)
Immunofluorescence (1:500)
Immunoprecipitation (2-5 ug/mg lysate)
The optimal working dilution should be determined by the end user.
It is not recommended for Western blot analysis.Storage Buffer
In PBS (0.02% sodium azide).
Storage Instruction
Store at 4°C for short term. For long term storage, aliquot and store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Immunocytochemistry
Immunofluorescence
Immunofluorescent staining of CIC polyclonal antibody (Cat # PAB12457) was tested in Neuro 2A cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).Immunoprecipitation
Detection of Human CIC/Capicua by Western blot of Immunoprecipitates. Whole cell lysate from HeLa cells. CIC polyclonal antibody (Cat # PAB12457) used for IP at 3 mcg/mg lysate. CIC was also IPed by rabbit anti-CICa antibody. -
Gene Info — CIC
Entrez GeneID
23152Protein Accession#
NP_055940.3; Q96RK0Gene Name
CIC
Gene Alias
KIAA0306
Gene Description
capicua homolog (Drosophila)
Gene Ontology
HyperlinkOther Designations
capicua homolog
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Interactome
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Disease
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