Rabbit polyclonal antibody raised against partial recombinant FANCD2.
Recombinant protein corresponding to N-terminus of human FANCD2.
This antibody is specific to FANCD2.
Quality Control Testing:
Antibody Reactive Against Recombinant Protein.
Immunofluorescence (1:200-1:500) Western Blot (1:10000-1:20000) Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (2.5-5 ug/mL) The optimal working dilution should be determined by the end user.
In Tris-glycine, 150 mM NaCl (0.05% sodium azide)
Store at -20°C or -80°C. Aliquot to avoid repeated freezing and thawing.
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Staining of human prostate, glandular epithelium using FANCD2 polyclonal antibody (Cat # PAB12428).
FANCD2 colocalizes in vivo with another protein in SiHa cells after cell exposure to IR. Proliferating SiHa cells were exposed to 10 Gy of IR and double -color immunofluorescence staining was performed after 8 h. FANCD2 were stained with FANCD2 polyclonal antibody (Cat # PAB12428).
FANCD2 polyclonal antibody (Cat # PAB12428) was used to stain FANCD2. FANCD2 colocalizes in vivo with another protein in U-2 OS cells after cell exposure to IR. Proliferating U-2 OS cells were exposed to 10 Gy of IR and double -color immunofluorescence staining was performed after 8 h.
The Fanconi anemia complementation group (FANC) currently includes FANCA, FANCB, FANCC, FANCD1 (also called BRCA2), FANCD2, FANCE, FANCF, FANCG, FANCI, FANCJ (also called BRIP1), FANCL, FANCM and FANCN (also called PALB2). The previously defined group FANCH is the same as FANCA. Fanconi anemia is a genetically heterogeneous recessive disorder characterized by cytogenetic instability, hypersensitivity to DNA crosslinking agents, increased chromosomal breakage, and defective DNA repair. The members of the Fanconi anemia complementation group do not share sequence similarity; they are related by their assembly into a common nuclear protein complex. This gene encodes the protein for complementation group D2. This protein is monoubiquinated in response to DNA damage, resulting in its localization to nuclear foci with other proteins (BRCA1 AND BRCA2) involved in homology-directed DNA repair. Alternative splicing results in two transcript variants encoding different isoforms. [provided by RefSeq
Fanconi anemia complementation group D2,type 4 Fanconi pancytopenia