FANCD2 polyclonal antibody
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More Files
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Western Blot (Cell lysate)
Western blot analysis of FANCD2 in HeLa whole cell extract using FANCD2 polyclonal antibody (Cat # PAB12428).
Immunohistochemistry
Staining of human prostate, glandular epithelium using FANCD2 polyclonal antibody (Cat # PAB12428).
Immunofluorescence
FANCD2 colocalizes in vivo with another protein in SiHa cells after cell exposure to IR. Proliferating SiHa cells were exposed to 10 Gy of IR and double -color immunofluorescence staining was performed after 8 h. FANCD2 were stained with FANCD2 polyclonal antibody (Cat # PAB12428).
Immunofluorescence
FANCD2 polyclonal antibody (Cat # PAB12428) was used to stain FANCD2. FANCD2 colocalizes in vivo with another protein in U-2 OS cells after cell exposure to IR. Proliferating U-2 OS cells were exposed to 10 Gy of IR and double -color immunofluorescence staining was performed after 8 h.
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Specification
Product Description
Rabbit polyclonal antibody raised against partial recombinant FANCD2.
Immunogen
Recombinant protein corresponding to N-terminus of human FANCD2.
Host
Rabbit
Reactivity
Human, Mouse
Specificity
This antibody is specific to FANCD2.
Form
Liquid
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Recommend Usage
Immunofluorescence (1:200-1:500)
Western Blot (1:10000-1:20000)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (2.5-5 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris-glycine, 150 mM NaCl (0.05% sodium azide)
Storage Instruction
Store at -20°C or -80°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of FANCD2 in HeLa whole cell extract using FANCD2 polyclonal antibody (Cat # PAB12428).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry
Staining of human prostate, glandular epithelium using FANCD2 polyclonal antibody (Cat # PAB12428).Immunofluorescence
FANCD2 colocalizes in vivo with another protein in SiHa cells after cell exposure to IR. Proliferating SiHa cells were exposed to 10 Gy of IR and double -color immunofluorescence staining was performed after 8 h. FANCD2 were stained with FANCD2 polyclonal antibody (Cat # PAB12428).Immunofluorescence
FANCD2 polyclonal antibody (Cat # PAB12428) was used to stain FANCD2. FANCD2 colocalizes in vivo with another protein in U-2 OS cells after cell exposure to IR. Proliferating U-2 OS cells were exposed to 10 Gy of IR and double -color immunofluorescence staining was performed after 8 h.Immunoprecipitation
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Gene Info — FANCD2
Entrez GeneID
2177Protein Accession#
Q9BXW9Gene Name
FANCD2
Gene Alias
DKFZp762A223, FA-D2, FA4, FACD, FAD, FAD2, FANCD, FLJ23826
Gene Description
Fanconi anemia, complementation group D2
Omim ID
227646Gene Ontology
HyperlinkGene Summary
The Fanconi anemia complementation group (FANC) currently includes FANCA, FANCB, FANCC, FANCD1 (also called BRCA2), FANCD2, FANCE, FANCF, FANCG, FANCI, FANCJ (also called BRIP1), FANCL, FANCM and FANCN (also called PALB2). The previously defined group FANCH is the same as FANCA. Fanconi anemia is a genetically heterogeneous recessive disorder characterized by cytogenetic instability, hypersensitivity to DNA crosslinking agents, increased chromosomal breakage, and defective DNA repair. The members of the Fanconi anemia complementation group do not share sequence similarity; they are related by their assembly into a common nuclear protein complex. This gene encodes the protein for complementation group D2. This protein is monoubiquinated in response to DNA damage, resulting in its localization to nuclear foci with other proteins (BRCA1 AND BRCA2) involved in homology-directed DNA repair. Alternative splicing results in two transcript variants encoding different isoforms. [provided by RefSeq
Other Designations
Fanconi anemia complementation group D2|type 4 Fanconi pancytopenia
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Interactome
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Disease
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Publication Reference
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Defective mitochondrial peroxiredoxin-3 results in sensitivity to oxidative stress in Fanconi anemia.
Mukhopadhyay SS, Leung KS, Hicks MJ, Hastings PJ, Youssoufian H, Plon SE.
The Journal of Cell Biology 2006 Oct; 175(2):225.
Application：WB, Human, HeLa cells.
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Young patients with oral squamous cell carcinoma: study of the involvement of GSTP1 and deregulation of the Fanconi anemia genes.
Tremblay S, Pintor Dos Reis P, Bradley G, Galloni NN, Perez-Ordonez B, Freeman J, Brown D, Gilbert R, Gullane P, Irish J, Kamel-Reid S.
Archives of Otolaryngology--Head & Neck Surgery 2006 Sep; 132(9):958.
Application：IHC-P, Human, Human oral squamous cell carcinoma.
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Spatial organization of the mammalian genome surveillance machinery in response to DNA strand breaks.
Bekker-Jensen S, Lukas C, Kitagawa R, Melander F, Kastan MB, Bartek J, Lukas J.
The Journal of Cell Biology 2006 Apr; 173(2):195.
Application：IF, Human, Human fibroblasts, U-2 OS cells.
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Nbs1 is required for ATR-dependent phosphorylation events.
Stiff T, Reis C, Alderton GK, Woodbine L, O'Driscoll M, Jeggo PA.
The EMBO Journal 2005 Jan; 24(1):199.
Application：WB-Ce, Human, NBS cells.
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Repair kinetics of genomic interstrand DNA cross-links: evidence for DNA double-strand break-dependent activation of the Fanconi anemia/BRCA pathway.
Rothfuss A, Grompe M.
Molecular and Cellular Biology 2004 Jan; 24(1):123.
Application：IF, WB, Human, PD797.f cells.
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Defective mitochondrial peroxiredoxin-3 results in sensitivity to oxidative stress in Fanconi anemia.
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