HIF1A polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against partial recombinant HIF1A.
Immunogen
Recombinant protein corresponding to amino acids 432-528 of human HIF1A.
Host
Rabbit
Reactivity
Human, Mouse, Primates, Rat
Specificity
This antibody is specific to HIF-1 alpha.
Form
Liquid
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Recommend Usage
Western Blot (1:500-1:1000)
Immunohistochemistry (1:100)
ChIP (25 ul/1.5 x 106 cells)
The optimal working dilution should be determined by the end user.Storage Buffer
In antiserum (0.05% sodium azide)
Storage Instruction
Store at 4°C for short term storage. For long term storage, store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
ChIP
Western Blot
Immunohistochemistry
Staining of HIF1A on human kidney, renal tubular epithelium in Cortex using HIF1A polyclonal antibody (Cat # PAB12418). -
Gene Info — HIF1A
Entrez GeneID
3091Protein Accession#
Q16665Gene Name
HIF1A
Gene Alias
HIF-1alpha, HIF1, HIF1-ALPHA, MOP1, PASD8, bHLHe78
Gene Description
hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor)
Omim ID
603348Gene Ontology
HyperlinkGene Summary
Hypoxia-inducible factor-1 (HIF1) is a transcription factor found in mammalian cells cultured under reduced oxygen tension that plays an essential role in cellular and systemic homeostatic responses to hypoxia. HIF1 is a heterodimer composed of an alpha subunit and a beta subunit. The beta subunit has been identified as the aryl hydrocarbon receptor nuclear translocator (ARNT). This gene encodes the alpha subunit of HIF-1. Overexpression of a natural antisense transcript (aHIF) of this gene has been shown to be associated with nonpapillary renal carcinomas. Two alternative transcripts encoding different isoforms have been identified. [provided by RefSeq
Other Designations
ARNT interacting protein|hypoxia-inducible factor 1, alpha subunit|hypoxia-inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor)|member of PAS superfamily 1
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Interactome
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Pathway
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Disease
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Publication Reference
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Stabilization of HIF-1alpha is critical to improve wound healing in diabetic mice.
Botusan IR, Sunkari VG, Savu O, Catrina AI, Grunler J, Lindberg S, Pereira T, Yla-Herttuala S, Poellinger L, Brismar K, Catrina SB.
PNAS 2008 Dec; 105(49):19426.
Application:WB-Ce, Human, Mouse, Human dermal fibroblast cells, Mouse primary fibroblasts.
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Population origin, development and temperature of development affect the amounts of HSP70, HSP90 and the putative hypoxia-inducible factor in the tadpoles of the common frog Rana temporaria.
Nikinmaa M, Leveelahti L, Dahl E, Rissanen E, Rytkonen KT, Laurila A.
The Journal of Experimental Biology 2008 Jun; 211(Pt 12):1999.
Application:WB, Frog, Nuclear extracts from tadpole.
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Transcriptional Regulation of SDHa flavoprotein by nuclear respiratory factor-1 prevents pseudo-hypoxia in aerobic cardiac cells.
Piantadosi CA, Suliman HB.
The Journal of Biological Chemistry 2008 Apr; 283(16):10967.
Application:IP, WB, Rat, H9c2 cells, Rat cardiomyocytes.
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Identification of small molecules from human embryonic stem cells using metabolomics.
Cezar GG, Quam JA, Smith AM, Rosa GJ, Piekarczyk MS, Brown JF, Gage FH, Muotri AR.
Stem Cells and Development 2007 Dec; 16(6):869.
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Redox regulation of lung development and perinatal lung epithelial function.
Land SC, Wilson SM.
Antioxidants & Redox Signaling 2005 Jan; 7(1-2):92.
Application:IHC, WB, Human, Cancers, Mammalian cells.
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Stabilization of HIF-1alpha is critical to improve wound healing in diabetic mice.
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