KIF11 polyclonal antibody
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More Files
- More Functions
Western Blot (Transfected lysate)
Western blot analysis of KIF11 on lysates of cells that were transfected with the pCMV6-ENTRY control or pCMV6-ENTRY KIF11 cDNA using KIF11 polyclonal antibody (Cat # PAB12408).
Immunofluorescence
Staining of KIF11 on HeLa cells fixed in 3.5% paraformaldehyde using KIF11 polyclonal antibody (Cat # PAB12408). Cytoplasmic staining during interphase and spindle staining during mitosis.
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Specification
Product Description
Rabbit polyclonal antibody raised against partial recombinant KIF11.
Immunogen
Recombinant protein corresponding to coiled-coil region of human KIF11.
Host
Rabbit
Reactivity
Human, Mammals, Mouse, Pig, Primates, Rat
Specificity
This antibody is specific to Eg5.
Form
Liquid
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Recommend Usage
Immunofluorescence (1:1000)
Immunoprecipitation (1:1000)
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In antiserum
Storage Instruction
Store at -20°C or -80°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Western Blot (Transfected lysate)
Western blot analysis of KIF11 on lysates of cells that were transfected with the pCMV6-ENTRY control or pCMV6-ENTRY KIF11 cDNA using KIF11 polyclonal antibody (Cat # PAB12408).Immunofluorescence
Staining of KIF11 on HeLa cells fixed in 3.5% paraformaldehyde using KIF11 polyclonal antibody (Cat # PAB12408). Cytoplasmic staining during interphase and spindle staining during mitosis.Immunoprecipitation
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Gene Info — KIF11
Entrez GeneID
3832Protein Accession#
P52732Gene Name
KIF11
Gene Alias
EG5, HKSP, KNSL1, TRIP5
Gene Description
kinesin family member 11
Omim ID
148760Gene Ontology
HyperlinkGene Summary
This gene encodes a motor protein that belongs to the kinesin-like protein family. Members of this protein family are known to be involved in various kinds of spindle dynamics. The function of this gene product includes chromosome positioning, centrosome separation and establishing a bipolar spindle during cell mitosis. [provided by RefSeq
Other Designations
OTTHUMP00000020099|kinesin-like 1|kinesin-like spindle protein|kinesin-related motor protein|thyroid receptor interacting protein 5
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Interactome
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Disease
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Publication Reference
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Prophase microtubule arrays undergo flux-like behavior in mammalian cells.
Ferenz NP, Wadsworth P.
Molecular Biology of the Cell 2007 Oct; 18(10):3993.
Application：IF, Human, LLC-Pk1-PA cells.
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Prophase microtubule arrays undergo flux-like behavior in mammalian cells.
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