MAT2A polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of MAT2A.
Immunogen
A synthetic peptide corresponding to amino acids 100-200 of human MAT2A.
Host
Rabbit
Reactivity
Bovine, Human, Primates, Rat, Zebra fish
Specificity
This antibody has 100% homology to both the MAT1A and MAT2A proteins. This antibody recognizes recombinant MAT2A protein, but specificity for MAT1A has not been tested.
Form
Liquid
Recommend Usage
Western Blot (2 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (30% glycerol, 0.09% sodium azide)
Storage Instruction
Store at 4°C for short term. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of MAT2A in HepG2 whole cell lysates using MAT2A polyclonal antibody (Cat # PAB12387). -
Gene Info — MAT2A
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Interactome
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Pathway
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Disease
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Publication Reference
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Leptin's mitogenic effect in human liver cancer cells requires induction of both methionine adenosyltransferase 2A and 2beta.
Ramani K, Yang H, Xia M, Ara AI, Mato JM, Lu SC.
Hepatology 2008 Feb; 47(2):521.
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Silencing MAT2A gene by RNA interference inhibited cell growth and induced apoptosis in human hepatoma cells.
Liu Q, Wu K, Zhu Y, He Y, Wu J, Liu Z.
Hepatology Research 2007 May; 37(5):376.
Application:WB-Tr, Human, Bel-7402, HepG2, Hep3B cells.
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Transcription of the MAT2A gene, coding for methionine adenosyltransferase, is up-regulated by E2F and Sp1 at a chromatin level during proliferation of liver cells.
Rodriguez JL, Boukaba A, Sandoval J, Georgieva EI, Latasa MU, Garcia-Trevijano ER, Serviddio G, Nakamura T, Avila MA, Sastre J, Torres L, Mato JM, Lopez-Rodas G.
The International Journal of Biochemistry & Cell Biology 2007 Jan; 39(4):842.
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Leptin's mitogenic effect in human liver cancer cells requires induction of both methionine adenosyltransferase 2A and 2beta.
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