NCL polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Detection of NCL in crude PD31 nuclear extracts using NCL polyclonal antibody (Cat # PAB123761).
Immunohistochemistry
Detection of NCL in human tonsil germinal centers using NCL polyclonal antibody (Cat # PAB12376).
Immunofluorescence
In-situ immunofluorescent staining of NCL in PD31 murine pre-B cells using NCL polyclonal antibody (Cat # PAB12376).
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Specification
Product Description
Rabbit polyclonal antibody raised against partial recombinant NCL.
Immunogen
Recombinant protein corresponding to amino acids 284-709 of human NCL.
Host
Rabbit
Reactivity
Human, Mouse
Specificity
This antibody is specific to nucleolin protein.
Form
Liquid
Recommend Usage
Immunofluorescence (1:50-1:100)
Western Blot (1:4000)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:50-1:100)
The optimal working dilution should be determined by the end user.Storage Buffer
In antiserum (0.09% sodium azide)
Storage Instruction
Store at -20°C or -80°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Detection of NCL in crude PD31 nuclear extracts using NCL polyclonal antibody (Cat # PAB123761).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry
Detection of NCL in human tonsil germinal centers using NCL polyclonal antibody (Cat # PAB12376).Immunofluorescence
In-situ immunofluorescent staining of NCL in PD31 murine pre-B cells using NCL polyclonal antibody (Cat # PAB12376). -
Gene Info — NCL
Entrez GeneID
4691Protein Accession#
P19338Gene Name
NCL
Gene Alias
C23, FLJ45706
Gene Description
nucleolin
Omim ID
164035Gene Ontology
HyperlinkGene Summary
Nucleolin (NCL), a eukaryotic nucleolar phosphoprotein, is involved in the synthesis and maturation of ribosomes. It is located mainly in dense fibrillar regions of the nucleolus. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. The intron 11 of the NCL gene encodes a small nucleolar RNA, termed U20. [provided by RefSeq
Other Designations
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Interactome
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Pathway
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Publication Reference
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Chemical proteomics identifies heterogeneous nuclear ribonucleoprotein (hnRNP) A1 as the molecular target of Quercetin in its anti-cancer effects in PC-3 cells.
Ko CC, Chen YJ, Chen CT, Liu YC, Cheng FC, Hsu KC, Chow LP.
The Journal of Biological Chemistry 2014 Aug; 289(32):22078.
Application：WB-Ce, Human, PC-3 cells.
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Selenoprotein H is a nucleolar thioredoxin-like protein with a unique expression pattern.
Novoselov SV, Kryukov GV, Xu XM, Carlson BA, Hatfield DL, Gladyshev VN.
The Journal of Biological Chemistry 2007 Apr; 282(16):11960.
Application：IF, WB, Mouse, LCC1, NIH 3T3 cells.
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Identification of novel proteins associated with both alpha-synuclein and DJ-1.
Jin J, Li GJ, Davis J, Zhu D, Wang Y, Pan C, Zhang J.
Molecular & Cellular Proteomics 2007 May; 6(5):845.
Application：IF, WB, Rat, Rat mesencephalic neuronal cell line.
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Up-regulation of the KLF2 transcription factor by fluid shear stress requires nucleolin.
Huddleson JP, Ahmad N, Lingrel JB.
The Journal of Biological Chemistry 2006 Jun; 281(22):15121.
Application：ChIP, IP, WB, Human, Mouse, EOMA, HUVEC, WR19M.1 cells, mouse macrophage cells.
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Nucleolin is one component of the B cell-specific transcription factor and switch region binding protein, LR1.
Hanakahi LA, Dempsey LA, Li MJ, Maizels N.
PNAS 1997 Apr; 94(8):3605.
Application：IF, WB-Ce, Mouse, PD31 murine pre-B cells.
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Chemical proteomics identifies heterogeneous nuclear ribonucleoprotein (hnRNP) A1 as the molecular target of Quercetin in its anti-cancer effects in PC-3 cells.
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