ROCK2 polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of ROCK2.
Immunogen
A synthetic peptide corresponding to amino acids at N-terminus of human ROCK2.
Host
Rabbit
Theoretical MW (kDa)
160.9
Reactivity
Human, Mouse, Rat
Specificity
Identical to the related rat and mouse sequence.
Form
Lyophilized
Purification
Affinity purification
Isotype
IgG
Recommend Usage
Western Blot (1 ug/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (2 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from 0.9 mg NaCl, 0.2 mg Na2HPO4 (5 mg BSA, 0.05 mg sodium azide, 0.05 mg Thimerosal)
Storage Instruction
Store at -20°C on dry atmosphere.
After reconstitution with 200 uL of deionized water and concentration will be 500 ug/mL, store at -20°C or lower.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide and thimerosal: POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western Blot analysis of ROCK2 expression from cell extracts with ROCK2 polyclonal antibody (Cat # PAB12328).
Lane 1 : MCF-7 whole cell lysate.
Lane 2 : HeLa whole cell lysate.
Lane 3 : smmc whole cell lysate.
Lane 4 : HT1080 whole cell lysate.
Lane 3 : MM453 whole cell lysate.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of ROCK2 on formalin fixed, paraffin embedded human intestinal cancer with ROCK2 polyclonal antibody (Cat # PAB12328). -
Gene Info — ROCK2
Entrez GeneID
9475Gene Name
ROCK2
Gene Alias
KIAA0619
Gene Description
Rho-associated, coiled-coil containing protein kinase 2
Omim ID
604002Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a serine/threonine kinase that regulates cytokinesis, smooth muscle contraction, the formation of actin stress fibers and focal adhesions, and the activation of the c-fos serum response element. This protein, which is an isozyme of ROCK1 is a target for the small GTPase Rho. [provided by RefSeq
Other Designations
OTTHUMP00000195104
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Interactome
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Pathway
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Disease
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Publication Reference
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Targeted disruption of the mouse rho-associated kinase 2 gene results in intrauterine growth retardation and fetal death.
Thumkeo D, Keel J, Ishizaki T, Hirose M, Nonomura K, Oshima H, Oshima M, Taketo MM, Narumiya S.
Molecular and Cellular Biology 2003 Jul; 23(14):5043.
Application:WB-Ti, Mouse, Brains, Embryos.
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Localization of the gene coding for ROCK II/Rho kinase on human chromosome 2p24.
Takahashi N, Tuiki H, Saya H, Kaibuchi K.
Genomics 1999 Jan; 55(2):235.
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Targeted disruption of the mouse rho-associated kinase 2 gene results in intrauterine growth retardation and fetal death.
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