KRT8 polyclonal antibody
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More Files
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of KRT8.
Immunogen
A synthetic peptide corresponding to amino acids at N-terminus of human KRT8.
Host
Rabbit
Theoretical MW (kDa)
53.7
Reactivity
Human
Specificity
Different to the related rat sequence by a single amino acid.
Form
Lyophilized
Purification
Affinity purification
Isotype
IgG
Recommend Usage
Western Blot (1 ug/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (2 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from 0.9 mg NaCl, 0.2 mg Na2HPO4 (5 mg BSA, 0.05 mg sodium azide, 0.05 mg Thimerosal)
Storage Instruction
Store at -20°C on dry atmosphere.
After reconstitution with 200 uL of deionized water and concentration will be 500 ug/mL, store at -20°C or lower.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide and thimerosal: POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western Blot analysis of KRT8 expression from cell extracts with KRT8 polyclonal antibody (Cat # PAB12326).
Lane 1 : HeLa whole cell lysate.
Lane 2 : HT1080 whole cell lysate.
Lane 3 : Colo 320 whole cell lysate.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of KRT8 on formalin fixed, paraffin embedded human lung cancer with KRT8 polyclonal antibody (Cat # PAB12326).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of KRT8 on formalin fixed, paraffin embedded human stomach cancer with KRT8 polyclonal antibody (Cat # PAB12326). -
Gene Info — KRT8
Entrez GeneID
3856Gene Name
KRT8
Gene Alias
CARD2, CK8, CYK8, K2C8, K8, KO
Gene Description
keratin 8
Gene Ontology
HyperlinkGene Summary
This gene is a member of the type II keratin family clustered on the long arm of chromosome 12. Type I and type II keratins heteropolymerize to form intermediate-sized filaments in the cytoplasm of epithelial cells. The product of this gene typically dimerizes with keratin 18 to form an intermediate filament in simple single-layered epithelial cells. This protein plays a role in maintaining cellular structural integrity and also functions in signal transduction and cellular differentiation. Mutations in this gene cause cryptogenic cirrhosis. [provided by RefSeq
Other Designations
cytokeratin 8|keratin, type II cytoskeletal 8
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Interactome
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Disease
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Publication Reference
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The intermediate filament protein keratin 8 is a novel cytoplasmic substrate for c-Jun N-terminal kinase.
He T, Stepulak A, Holmstrom TH, Omary MB, Eriksson JE.
The Journal of Biological Chemistry 2002 Mar; 277(13):10767.
Application:WB, Human, HT-29 cells.
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Cloning and sequence of cDNA for human placental cytokeratin 8. Regulation of the mRNA in trophoblastic cells by cAMP.
Yamamoto R, Kao LC, McKnight CE, Strauss JF 3rd.
Molecular Endocrinology (Baltimore, Md.) 1990 Mar; 4(3):370.
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Organization and sequence of the human gene encoding cytokeratin 8.
Krauss S, Franke WW.
Gene 1990 Feb; 86(2):241.
Application:IF, Bovine, Dog, Rat, MDBK cells, MDCK cells, Rat hepatoma cells MH1C1.
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The intermediate filament protein keratin 8 is a novel cytoplasmic substrate for c-Jun N-terminal kinase.
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