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Last updated: 2016/12/4
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GFAP polyclonal antibody

  • Catalog # : PAB12325
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic peptide of GFAP.
  • Immunogen:
  • A synthetic peptide corresponding to amino acids at C-terminus of human GFAP.
  • Host:
  • Rabbit
  • Theoretical MW (kDa):
  • 49, 50, 49.5
  • Reactivity:
  • Human, Mouse, Rat
  • Specificity:
  • Identical to the related rat and mouse sequence.
  • Form:
  • Lyophilized
  • Purification:
  • Affinity purification
  • Isotype:
  • IgG
  • Recommend Usage:
  • Western Blot (1 ug/mL)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (2 ug/mL)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • Lyophilized from 0.9 mg NaCl, 0.2 mg Na2HPO4 (5 mg BSA, 0.05 mg sodium azide, 0.05 mg Thimerosal)
  • Storage Instruction:
  • Store at -20°C on dry atmosphere.
    After reconstitution with 200 uL of deionized water and concentration will be 500 ug/mL, store at -20°C or lower.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide and thimerosal: POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Tissue lysate)
  • Western Blot (Tissue lysate)
  • Western Blot analysis of GFAP expression from cell extracts with GFAP polyclonal antibody (Cat # PAB12325).
    Lane 1 : rat brain tissue lysate.
    Lane 2 : rat brain tissue lysate.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemical staining of GFAP on formalin fixed, paraffin embedded human brain tissue with GFAP polyclonal antibody (Cat # PAB12325).
  • Application Image
  • Western Blot (Tissue lysate)
  • Western Blot (Tissue lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • Gene Information
  • Entrez GeneID:
  • 2670
  • Gene Name:
  • GFAP
  • Gene Alias:
  • FLJ45472
  • Gene Description:
  • glial fibrillary acidic protein
  • Gene Summary:
  • This gene encodes one of the major intermediate filament proteins of mature astrocytes. It is used as a marker to distinguish astrocytes from other glial cells during development. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq
  • Other Designations:
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