Western Blot analysis of XAF1 expression from cell and tissue extracts with XAF1 polyclonal antibody (Cat # PAB12307). Lane 1 : rat liver tissue lysate. Lane 2 : rat kidney tissue lysate. Lane 3 : rat spleen tissue lysate. Lane 4 : human placenta tissue lysate. Lane 5 : Marker. Lane 6 : MCF-7 whole cell lysate. Lane 7 : HeLa whole cell lysate. Lane 8 : smmc cell lysate. Lane 9 : HT1080 whole cell lysate. Lane 10 : Colo 320 whole cell lysate.
X-linked inhibitor of apoptosis (XIAP; MIM 300079) is a potent member of the IAP family. All members of this family possess baculoviral IAP (BIR) repeats, cysteine-rich domains of approximately 80 amino acids that bind and inhibit caspases (e.g., CASP3; MIM 600636). XIAP has 3 BIR domains and a C-terminal RING zinc finger that possesses E3 ubiquitin ligase (see MIM 601623) activity. XAF1 antagonizes the anticaspase activity of XIAP and may be important in mediating apoptosis resistance in cancer cells (Liston et al., 2001 [PubMed 11175744]).[supplied by OMIM