XAF1 polyclonal antibody
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More Files
- More Functions
Western Blot
Western Blot analysis of XAF1 expression from cell and tissue extracts with XAF1 polyclonal antibody (Cat # PAB12307).
Lane 1 : rat liver tissue lysate.
Lane 2 : rat kidney tissue lysate.
Lane 3 : rat spleen tissue lysate.
Lane 4 : human placenta tissue lysate.
Lane 5 : Marker.
Lane 6 : MCF-7 whole cell lysate.
Lane 7 : HeLa whole cell lysate.
Lane 8 : smmc cell lysate.
Lane 9 : HT1080 whole cell lysate.
Lane 10 : Colo 320 whole cell lysate.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of XAF1 on formalin fixed, paraffin embedded human epidermic carcinoma with XAF1 polyclonal antibody (Cat # PAB12307).
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of XAF1.
Immunogen
A synthetic peptide corresponding to amino acids at C-terminus of human XAF1.
Host
Rabbit
Theoretical MW (kDa)
34.6
Reactivity
Human, Mouse, Rat
Specificity
Identical to the related rat and mouse sequence.
Form
Lyophilized
Purification
Affinity purification
Isotype
IgG
Recommend Usage
Western Blot (1 ug/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (0.5-1 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from 0.9 mg NaCl, 0.2 mg Na2HPO4 (5 mg BSA, 0.05 mg sodium azide, 0.05 mg Thimerosal)
Storage Instruction
Store at -20°C on dry atmosphere.
After reconstitution with 200 uL of deionized water and concentration will be 500 ug/mL, store at -20°C or lower.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide and thimerosal: POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western Blot analysis of XAF1 expression from cell and tissue extracts with XAF1 polyclonal antibody (Cat # PAB12307).
Lane 1 : rat liver tissue lysate.
Lane 2 : rat kidney tissue lysate.
Lane 3 : rat spleen tissue lysate.
Lane 4 : human placenta tissue lysate.
Lane 5 : Marker.
Lane 6 : MCF-7 whole cell lysate.
Lane 7 : HeLa whole cell lysate.
Lane 8 : smmc cell lysate.
Lane 9 : HT1080 whole cell lysate.
Lane 10 : Colo 320 whole cell lysate.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of XAF1 on formalin fixed, paraffin embedded human epidermic carcinoma with XAF1 polyclonal antibody (Cat # PAB12307). -
Gene Info — XAF1
Entrez GeneID
54739Gene Name
XAF1
Gene Alias
BIRC4BP, HSXIAPAF1, XIAPAF1
Gene Description
XIAP associated factor 1
Omim ID
606717Gene Ontology
HyperlinkGene Summary
X-linked inhibitor of apoptosis (XIAP; MIM 300079) is a potent member of the IAP family. All members of this family possess baculoviral IAP (BIR) repeats, cysteine-rich domains of approximately 80 amino acids that bind and inhibit caspases (e.g., CASP3; MIM 600636). XIAP has 3 BIR domains and a C-terminal RING zinc finger that possesses E3 ubiquitin ligase (see MIM 601623) activity. XAF1 antagonizes the anticaspase activity of XIAP and may be important in mediating apoptosis resistance in cancer cells (Liston et al., 2001 [PubMed 11175744]).[supplied by OMIM
Other Designations
BIRC4 binding protein
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Interactome
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Publication Reference
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Identification of XAF1 as an antagonist of XIAP anti-Caspase activity.
Liston P, Fong WG, Kelly NL, Toji S, Miyazaki T, Conte D, Tamai K, Craig CG, McBurney MW, Korneluk RG.
Nature Cell Biology 2001 Feb; 3(2):128.
Application：WB-Ce, WB-Tr, Human, SF-295, SF-539, OVCAR-3, OVCAR-5 cells.
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Expression and genetic analysis of XIAP-associated factor 1 (XAF1) in cancer cell lines.
Fong WG, Liston P, Rajcan-Separovic E, St Jean M, Craig C, Korneluk RG.
Genomics 2000 Nov; 70(1):113.
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Identification of XAF1 as an antagonist of XIAP anti-Caspase activity.
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