NOTCH1 polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of NOTCH1.
Immunogen
A synthetic peptide corresponding to amino acids at internal region of human NOTCH1.
Host
Rabbit
Theoretical MW (kDa)
272
Reactivity
Human
Specificity
Different from the related rat sequence by one amino acid.
Form
Lyophilized
Purification
Affinity purification
Isotype
IgG
Recommend Usage
Western Blot (1 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from 0.9 mg NaCl, 0.2 mg Na2HPO4 (5 mg BSA, 0.05 mg sodium azide, 0.05 mg Thimerosal)
Storage Instruction
Store at -20°C on dry atmosphere.
After reconstitution with 200 uL of deionized water and concentration will be 500 ug/mL, store at -20°C or lower.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide and thimerosal: POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western Blot analysis of NOTCH1 expression from cell extracts with NOTCH1 polyclonal antibody (Cat # PAB12305).
Lane 1 : Marker.
Lane 2 : MCF-7 whole cell lysate.
Lane 3 : HeLa whole cell lysate.
Lane 4 : Jurkat whole cell lysate.
Lane 5 : Colo 320 whole cell lysate. -
Gene Info — NOTCH1
Entrez GeneID
4851Gene Name
NOTCH1
Gene Alias
TAN1, hN1
Gene Description
Notch homolog 1, translocation-associated (Drosophila)
Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the Notch family. Members of this Type 1 transmembrane protein family share structural characteristics including an extracellular domain consisting of multiple epidermal growth factor-like (EGF) repeats, and an intracellular domain consisting of multiple, different domain types. Notch family members play a role in a variety of developmental processes by controlling cell fate decisions. The Notch signaling network is an evolutionarily conserved intercellular signaling pathway which regulates interactions between physically adjacent cells. In Drosophilia, notch interaction with its cell-bound ligands (delta, serrate) establishes an intercellular signaling pathway that plays a key role in development. Homologues of the notch-ligands have also been identified in human, but precise interactions between these ligands and the human notch homologues remain to be determined. This protein is cleaved in the trans-Golgi network, and presented on the cell surface as a heterodimer. This protein functions as a receptor for membrane bound ligands, and may play multiple roles during development. [provided by RefSeq
Other Designations
OTTHUMP00000022594|neurogenic locus notch homolog protein 1|notch1|translocation-associated notch protein TAN-1
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Interactome
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Pathway
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Disease
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Publication Reference
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Notch1 and Notch3 instructively restrict bFGF-responsive multipotent neural progenitor cells to an astroglial fate.
Tanigaki K, Nogaki F, Takahashi J, Tashiro K, Kurooka H, Honjo T.
Neuron 2001 Jan; 29(1):45.
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Notch1 is required for the coordinate segmentation of somites.
Conlon RA, Reaume AG, Rossant J.
Development 1995 May; 121(5):1533.
Application:IHC, Mouse, Mouse embryos.
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Notch1 and Notch3 instructively restrict bFGF-responsive multipotent neural progenitor cells to an astroglial fate.
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