RELA polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of RELA.
Immunogen
A synthetic peptide corresponding to amino acids at N-terminus of human RELA.
Host
Rabbit
Theoretical MW (kDa)
60
Reactivity
Human
Specificity
Identical to the related rat and mouse sequence.
Form
Lyophilized
Purification
Affinity purification
Isotype
IgG
Recommend Usage
Western Blot (1 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from 0.9 mg NaCl, 0.2 mg Na2HPO4 (5 mg BSA, 0.05 mg sodium azide, 0.05 mg Thimerosal)
Storage Instruction
Store at -20°C on dry atmosphere.
After reconstitution with 200 uL of deionized water and concentration will be 500 ug/mL, store at -20°C or lower.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide and thimerosal: POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
Western Blot analysis of RELA expression from human colon carcinoma lyate with RELA polyclonal antibody (Cat # PAB12295). -
Gene Info — RELA
Entrez GeneID
5970GeneBank Accession#
BC033522Protein Accession#
AAH33522Gene Name
RELA
Gene Alias
MGC131774, NFKB3, p65
Gene Description
v-rel reticuloendotheliosis viral oncogene homolog A (avian)
Omim ID
164014Gene Ontology
HyperlinkGene Summary
NFKB1 (MIM 164011) or NFKB2 (MIM 164012) is bound to REL (MIM 164910), RELA, or RELB (MIM 604758) to form the NFKB complex. The p50 (NFKB1)/p65 (RELA) heterodimer is the most abundant form of NFKB. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA, MIM 164008 or NFKBIB, MIM 604495), which inactivate NFKB by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA, MIM 600664, or IKBKB, MIM 603258) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NFKB complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime (where H is A, C, or T; R is an A or G purine; and Y is a C or T pyrimidine).[supplied by OMIM
Other Designations
nuclear factor of kappa light polypeptide gene enhancer in B-cells 3|v-rel avian reticuloendotheliosis viral oncogene homolog A (nuclear factor of kappa light polypeptide gene enhancer in B-cells 3 (p65))|v-rel reticuloendotheliosis viral oncogene homolog
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Interactome
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Pathway
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Disease
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Publication Reference
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Activation of the transcription factor NF-kappaB in Schwann cells is required for peripheral myelin formation.
Nickols JC, Valentine W, Kanwal S, Carter BD.
Nature Neuroscience 2003 Feb; 6(2):161.
Application:IHC-P, IS, GSA, Rat, Sciatic nerves, Schwann cells.
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Phosphorylation of NF-kappa B p65 by PKA stimulates transcriptional activity by promoting a novel bivalent interaction with the coactivator CBP/p300.
Zhong H, Voll RE, Ghosh S.
Molecular Cell 1998 Apr; 1(5):661.
Application:IP, WB-Tr, Mouse, Monkey, 70Z/3, COS cells.
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Genomic organization of the gene encoding the p65 subunit of NF-kappa B: multiple variants of the p65 protein may be generated by alternative splicing.
Deloukas P, van Loon AP.
Human Molecular Genetics 1993 Nov; 2(11):1895.
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Activation of the transcription factor NF-kappaB in Schwann cells is required for peripheral myelin formation.
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