MMP16 polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of MMP16.
Immunogen
A synthetic peptide corresponding to amino acids at C-terminus of human MMP16.
Host
Rabbit
Theoretical MW (kDa)
69.5, 52.5
Reactivity
Human, Mouse, Rat
Specificity
Identical to the related rat sequence. This antibody recognizes 69.5 KDa for MMP16 long form and 52.5 KDa for short form.
Form
Lyophilized
Purification
Affinity purification
Isotype
IgG
Recommend Usage
Western Blot (1-2 ug/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (0.5-1 ug/mL)
Immunohistochemistry (Frozen sections) (0.5-1 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from 0.9 mg NaCl, 0.2 mg Na2HPO4 (5 mg BSA, 0.05 mg sodium azide, 0.05 mg Thimerosal)
Storage Instruction
Store at -20°C on dry atmosphere.
After reconstitution with 200 uL of deionized water and concentration will be 500 ug/mL, store at -20°C or lower.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide and thimerosal: POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western Blot analysis of MMP16 expression from HeLa cell lyate with MMP16 polyclonal antibody (Cat # PAB12293).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of MMP16 on formalin fixed, paraffin embedded rat kidney with MMP16 polyclonal antibody (Cat # PAB12293).Immunohistochemistry (Frozen sections)
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Gene Info — MMP16
Entrez GeneID
4325GeneBank Accession#
BC069500Protein Accession#
AAH69500Gene Name
MMP16
Gene Alias
C8orf57, MMP-X2, MT-MMP2, MT-MMP3, MT3-MMP
Gene Description
matrix metallopeptidase 16 (membrane-inserted)
Omim ID
602262Gene Ontology
HyperlinkGene Summary
Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. This gene produces at least two transcripts, one which encodes a membrane-bound form and another a soluble form of the protein. Both forms of the protein activate MMP2 by cleavage. This gene was once referred to as MT-MMP2, but was renamed as MT-MMP3 or MMP16. [provided by RefSeq
Other Designations
matrix metalloproteinase 16|matrix metalloproteinase 16 (membrane-inserted)|membrane-type matrix metalloproteinase 3|membrane-type-3 matrix metalloproteinase
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Disease
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Publication Reference
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Assignment of the human genes for membrane-type-1, -2, and -3 matrix metalloproteinases (MMP14, MMP15, and MMP16) to 14q12.2, 16q12.2-q21, and 8q21, respectively, by in situ hybridization.
Sato H, Tanaka M, Takino T, Inoue M, Seiki M.
Genomics 1997 Feb; 39(3):412.
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Identification of the second membrane-type matrix metalloproteinase (MT-MMP-2) gene from a human placenta cDNA library. MT-MMPs form a unique membrane-type subclass in the MMP family.
Takino T, Sato H, Shinagawa A, Seiki M.
The Journal of Biological Chemistry 1995 Sep; 270(39):23013.
Application:IP, WB-Re, Monkey, COS-1 cells.
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Assignment of the human genes for membrane-type-1, -2, and -3 matrix metalloproteinases (MMP14, MMP15, and MMP16) to 14q12.2, 16q12.2-q21, and 8q21, respectively, by in situ hybridization.
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