CTNNA1 polyclonal antibody
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More Files
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of CTNNA1.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to residues surrounding S641 of human CTNNA1.
Sequence
DDSDF
Host
Rabbit
Theoretical MW (kDa)
100
Reactivity
Human, Mouse
Specificity
alpha-catenin (Ab-641) antibody detects endogenous levels of total alpha-catenin protein.
Form
Liquid
Purification
Immunoaffinity purification
Concentration
1 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
Western Blot (1:500-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (without Mg2+ and Ca2+), 150 mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of extract from A-431 cells, untreated or treated with EGF (200ng/ml, 30min), using CTNNA1 polyclonal antibody (Cat # PAB12225, Lane 1 and 2). -
Gene Info — CTNNA1
Entrez GeneID
1495Protein Accession#
P35221Gene Name
CTNNA1
Gene Alias
CAP102, FLJ36832
Gene Description
catenin (cadherin-associated protein), alpha 1, 102kDa
Omim ID
116805Gene Ontology
HyperlinkGene Summary
alpha 1
Other Designations
alpha-E-catenin|alpha-catenin|alphaE-catenin|cadherin-associated protein,102kDa|catenin (cadherin-associated protein), alpha 1 (102kD)|catenin, alpha 1
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Interactome
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Pathway
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Disease
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Publication Reference
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Alpha-catenin is a molecular switch that binds E-cadherin-beta-catenin and regulates actin-filament assembly.
Drees F, Pokutta S, Yamada S, Nelson WJ, Weis WI.
Cell 2005 Dec; 123(5):903.
Application:WB, Chicken, Chicken pectoral muscles.
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Deconstructing the cadherin-catenin-actin complex.
Yamada S, Pokutta S, Drees F, Weis WI, Nelson WJ.
Cell 2005 Dec; 123(5):889.
Application:IF, Dog, MDCK GII cells.
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Regulation of beta-catenin signaling and maintenance of chondrocyte differentiation by ubiquitin-independent proteasomal degradation of alpha-catenin.
Hwang SG, Yu SS, Ryu JH, Jeon HB, Yoo YJ, Eom SH, Chun JS.
The Journal of Biological Chemistry 2005 Apr; 280(13):12758.
Application:IP, WB, Rabbit, Rabbit articular chondrocytes.
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Alpha-catenin is a molecular switch that binds E-cadherin-beta-catenin and regulates actin-filament assembly.
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