Rps6ka1 (phospho S352) polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of extract from HUVEC cells using Rps6ka1 (phospho S352) polyclonal antibody (Cat # PAB12168, Lane 1 and 2).
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic phosphopeptide of Rps6ka1.
Immunogen
Synthetic phosphopeptide (conjugated with KLH) corresponding to residues surrounding S352 of mouse Rps6ka1.
Sequence
RDSpPG
Host
Rabbit
Theoretical MW (kDa)
90
Reactivity
Human, Mouse, Rat
Specificity
p90RSK (Phospho-Ser352) antibody detects endogenous levels of p90RSK only when phosphorylated at serine 352.
Form
Liquid
Purification
Immunoaffinity purification
Concentration
1 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
Western Blot (1:500-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (without Mg2+ and Ca2+), 150 mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of extract from HUVEC cells using Rps6ka1 (phospho S352) polyclonal antibody (Cat # PAB12168, Lane 1 and 2). -
Gene Info — Rps6ka1
Entrez GeneID
20111Protein Accession#
P18653Gene Name
Rps6ka1
Gene Alias
Rsk1, p90rsk, rsk
Gene Description
ribosomal protein S6 kinase polypeptide 1
Gene Ontology
HyperlinkGene Summary
90kD
Other Designations
OTTMUSP00000010160|ribosomal protein S6 kinase, 90kD, polypeptide 1; Ribosomal protein S6 kinase, 90kD, 1
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Publication Reference
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Role of p90 ribosomal S6 kinase (p90RSK) in reactive oxygen species and protein kinase C beta (PKC-beta)-mediated cardiac troponin I phosphorylation.
Itoh S, Ding B, Bains CP, Wang N, Takeishi Y, Jalili T, King GL, Walsh RA, Yan C, Abe J.
The Journal of Biological Chemistry 2005 Jun; 280(25):24135.
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Activation of p90 Rsk1 is sufficient for differentiation of PC12 cells.
Silverman E, Frödin M, Gammeltoft S, Maller JL.
Molecular and Cellular Biology 2004 Dec; 24(24):10573.
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Mitogen-activated protein kinase pathway-dependent tumor-specific survival signaling in melanoma cells through inactivation of the proapoptotic protein bad.
Eisenmann KM, VanBrocklin MW, Staffend NA, Kitchen SM, Koo HM.
Cancer Research 2003 Dec; 63(23):8330.
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Role of p90 ribosomal S6 kinase (p90RSK) in reactive oxygen species and protein kinase C beta (PKC-beta)-mediated cardiac troponin I phosphorylation.
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