Lox polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Detection of Lox in MC3T3-E1 cell lysate using Lox polyclonal antibody (Cat # PAB12158).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Staining of Lox in mouse intestine using Lox polyclonal antibody (Cat # PAB12158).
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of Lox.
Immunogen
A synthetic peptide corresponding to amino acids 78-115 of mouse Lox.
Host
Rabbit
Reactivity
Human, Mouse, Pig, Primates, Rat
Specificity
This antibody recognizes the pro-enzyme (50 KDa) and pro-peptide. The pro-peptide runs at ~18-20 KDa when not glycosylated and ~35 KDa on SDS-PAGE when N-glycosylated.
Form
Liquid
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
Immunocytochemistry (1:50)
Immunofluorescence (1:50)
Immunohistochemistry (0.5-1 ug/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:100-1:250)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS
Storage Instruction
Store at 4°C for short term. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Western Blot (Cell lysate)
Detection of Lox in MC3T3-E1 cell lysate using Lox polyclonal antibody (Cat # PAB12158).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Staining of Lox in mouse intestine using Lox polyclonal antibody (Cat # PAB12158).Immunocytochemistry
Immunofluorescence
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Publication Reference
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Intracellular distribution of the lysyl oxidase propeptide in osteoblastic cells.
Guo Y, Pischon N, Palamakumbura AH, Trackman PC.
American Journal of Physiology. Cell Physiology 2007 Jun; 292(6):C2095.
Application：IF, WB, Mouse, MC3T3-E1 cells.
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The propeptide domain of lysyl oxidase induces phenotypic reversion of ras-transformed cells.
Palamakumbura AH, Jeay S, Guo Y, Pischon N, Sommer P, Sonenshein GE, Trackman PC.
The Journal of Biological Chemistry 2004 Sep; 279(39):40593.
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A role for lysyl oxidase regulation in the control of normal collagen deposition in differentiating osteoblast cultures.
Hong HH, Pischon N, Santana RB, Palamakumbura AH, Chase HB, Gantz D, Guo Y, Uzel MI, Ma D, Trackman PC.
Journal of Cellular Physiology 2004 Jul; 200(1):53.
Application：ICC, WB, Mouse, MC3T3-E1 cells.
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Intracellular distribution of the lysyl oxidase propeptide in osteoblastic cells.
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