Scarb2 polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of Scarb2.
Immunogen
A synthetic peptide corresponding to C-terminus of mouse Scarb2.
Host
Rabbit
Reactivity
Dog, Human, Mouse, Rat
Specificity
This antibody is specific to mouse and human LIMPII/lgp85.
Form
Liquid
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
Immunocytochemistry (1:250)
Immunofluorescence (1:50-1:250)
Immunohistochemistry (1:200)
Immunoprecipitation (1:100)
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In antiserum (0.05% sodium azide)
Storage Instruction
Store at -20°C or -80°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of Scarb2 in various samples using Scarb2 polyclonal antibody (Cat # PAB12157). ECL 40 minute exposure. Lane 1 : Rat cardiac fibroblasts (RCF). Lane 2 : 3T3 mouse fibroblasts. Lane 3 : Long hond canine lung lysate. Lane 4 : RCF treated with angiotensin II. Lane 5 : RCF treated with H2O2. Lane 6 : HEPG2 human liver lysate.Immunohistochemistry
Staining of Scarb2 in rat infarctzone tissue using Scarb2 polyclonal antibody (Cat # PAB12157) with a DAB detection system.Immunocytochemistry
Immunofluorescence
Immunoprecipitation
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Gene Info — Scarb2
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Publication Reference
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A nonsense mutation in the LIMP-2 gene associated with progressive myoclonic epilepsy and nephrotic syndrome.
Balreira A, Gaspar P, Caiola D, Chaves J, Beirao I, Lima JL, Azevedo JE, Miranda MC.
Human Molecular Genetics 2008 Apr; 17(14):2238.
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A nonsense mutation in the LIMP-2 gene associated with progressive myoclonic epilepsy and nephrotic syndrome.
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