Calr polyclonal antibody
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More Files
- More Functions
Immunofluorescence
Immunofluorescence staining of Calr in HCT15 colon cancer cells using Calr polyclonal antibody (Cat # PAB12147). Secondary antibody was an Alexa Fluor 488. Photo courtesy of Dr. Birkenkamp Demtroeder.
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Specification
Product Description
Rabbit polyclonal antibody raised against recombinant Calr.
Immunogen
Recombinant fusion protein corresponding to mouse Calr.
Host
Rabbit
Reactivity
Bovine, Human, Mouse, Rat
Specificity
This antibody is specific to calreticulin.
Form
Liquid
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Recommend Usage
Immunocytochemistry (1:250)
Immunofluorescence (1:50)
Immunohistochemistry (1:50)
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.05% sodium azide)
Storage Instruction
Store at -20°C or -80°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Immunohistochemistry
Immunocytochemistry
Immunofluorescence
Immunofluorescence staining of Calr in HCT15 colon cancer cells using Calr polyclonal antibody (Cat # PAB12147). Secondary antibody was an Alexa Fluor 488. Photo courtesy of Dr. Birkenkamp Demtroeder.Immunoprecipitation
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Gene Info — Calr
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Publication Reference
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Identification of human T cell leukemia virus type 1 tax amino acid signals and cellular factors involved in secretion of the viral oncoprotein.
Jain P, Mostoller K, Flaig KE, Ahuja J, Lepoutre V, Alefantis T, Khan ZK, Wigdahl B.
The Journal of Biological Chemistry 2007 Nov; 282(47):34581.
Application：Array, Mouse, BHK-21 cells.
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Impaired megakaryocytopoiesis in type 2B von Willebrand disease with severe thrombocytopenia.
Nurden P, Debili N, Vainchenker W, Bobe R, Bredoux R, Corvazier E, Combrie R, Fressinaud E, Meyer D, Nurden AT, Enouf J.
Blood 2006 Oct; 108(8):2587.
Application：WB-Ce, Human, Human megakaryocytes, platelets.
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ATP-sensitive K+-channel subunits on the mitochondria and endoplasmic reticulum of rat cardiomyocytes.
Zhou M, Tanaka O, Sekiguchi M, He HJ, Yasuoka Y, Itoh H, Kawahara K, Abe H.
The Journal of Histochemistry and Cytochemistry 2005 Dec; 53(12):1491.
Application：IF, WB-Ti, Rat, Heart.
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A splice variant of the TCR zeta mRNA lacking exon 7 leads to the down-regulation of TCR zeta, the TCR/CD3 complex, and IL-2 production in systemic lupus erythematosus T cells.
Tsuzaka K, Setoyama Y, Yoshimoto K, Shiraishi K, Suzuki K, Abe T, Takeuchi T.
The Journal of Immunology 2005 Mar; 174(6):3518.
Application：IF, Human, MA5.8 cells.
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Human FK506 binding protein 65 is associated with colorectal cancer.
Olesen SH, Christensen LL, Sørensen FB, Cabezón T, Laurberg S, Orntoft TF, Birkenkamp-Demtröder K.
Molecular & Cellular Proteomics 2005 Apr; 4(4):534.
Application：IF, Monkey, COS-7 cells.
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Identification of human T cell leukemia virus type 1 tax amino acid signals and cellular factors involved in secretion of the viral oncoprotein.
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