Fasn polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of Fasn.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to N-terminus of mouse Fasn.
Host
Rabbit
Reactivity
Chicken, Human, Mouse, Pig, Primates, Rat
Specificity
This antibody is specific to FAS protein.
Form
Liquid
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
Immunoprecipitation (1:100)
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In antiserum (0.05% sodium azide)
Storage Instruction
Store at -20°C or -80°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
Western blot analysis of Fasn in mouse liver lysate using Fasn polyclonal antibody (Cat # PAB12142). Samples : 50 ug of total mouse liver lysate.Western Blot (Tissue lysate)
Western blot analysis of Fasn in tissue lysates (brain, hypothalmus, and liver and muscle) using Fasn polyclonal antibody (Cat # PAB12142).Immunohistochemistry
Immunoprecipitation
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Gene Info — Fasn
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Publication Reference
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Mechanisms of antidiabetogenic and body weight-lowering effects of estrogen in high-fat diet-fed mice.
Bryzgalova G, Lundholm L, Portwood N, Gustafsson JA, Khan A, Efendic S, Dahlman-Wright K.
American Journal of Physiology. Endocrinology and Metabolism 2008 Oct; 295(4):E904.
Application:WB, Mouse , Mouse liver.
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1-11C-acetate as a PET radiopharmaceutical for imaging fatty acid synthase expression in prostate cancer.
Vavere AL, Kridel SJ, Wheeler FB, Lewis JS.
Journal of Nuclear Medicine: Official Publication, Society of Nuclear Medicine 2008 Jan; 49(2):327.
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Androgen deprivation increases p300 expression in prostate cancer cells.
Heemers HV, Sebo TJ, Debes JD, Regan KM, Raclaw KA, Murphy LM, Hobisch A, Culig Z, Tindall DJ.
Cancer Research 2007 Apr; 67(7):3422.
Application:WB-Tr, Human, LNCaP cells.
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Long-term administration of estradiol decreases expression of hepatic lipogenic genes and improves insulin sensitivity in ob/ob mice: a possible mechanism is through direct regulation of signal transducer and activator of transcription 3.
Gao H, Bryzgalova G, Hedman E, Khan A, Efendic S, Gustafsson JA, Dahlman-Wright K.
Molecular Endocrinology 2006 Jun; 20(6):1287.
Application:WB-Ti, Mouse, Livers.
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FAS inhibitor cerulenin reduces food intake and melanocortin receptor gene expression without modulating the other (an)orexigenic neuropeptides in chickens.
Dridi S, Ververken C, Hillgartner FB, Arckens L, Van der Gucht E, Cnops L, Decuypere E, Buyse J.
American Journal of Physiology. Regulatory, Integrative and Comparative Physiology 2006 Jul; 291(1):R138.
Application:IHC-P, WB-Ti, Chicken, Brain, Hypothalamus, Liver, Muscle.
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Mechanisms of antidiabetogenic and body weight-lowering effects of estrogen in high-fat diet-fed mice.
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