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Last updated: 2022/5/22

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Hif1a polyclonal antibody 

  • Catalog # : PAB12138
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against partial recombinant Hif1a.
  • Immunogen:
  • Recombinant protein corresponding to amino acids 530-825 of mouse Hif1a.
  • Host:
  • Rabbit
  • Reactivity:
  • Goat, Mouse
  • Specificity:
  • This antibody is specific to mouse HIF-1 alpha.
  • Form:
  • Liquid
  • Purification:
  • Immunogen affinity purification
  • Quality Control Testing:
  • Antibody Reactive Against Recombinant Protein.
  • Recommend Usage:
  • Chromatin Immunoprecipitation (1:10-1:500)
    Immunocytochemistry (1:100)
    Immunofluorescence (1:100)
    Immunohistochemistry (Frozen sections) (5-10 ug/mL)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (5-10 ug/mL)
    Western Blot (1-2 ug/mL)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS (0.02% sodium azide)
  • Storage Instruction:
  • Store at -20°C.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot analysis of Hif1a in a hypoxic sample using Hif1a polyclonal antibody (Cat # PAB12138). Lane 1 : CoCl2 treated COS-7 nuclear extract (50 ug, hypoxic). Lane 2 : Untreated COS-7 nuclear extract (50 ug, normoxic). 10 second ECL exposure.
  • Immunohistochemistry
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunohistochemistry
  • Gene Information
  • Gene Name:
  • Hif1a
  • Gene Alias:
  • AA959795,HIF-1alpha,HIF1alpha,MOP1
  • Gene Description:
  • hypoxia inducible factor 1, alpha subunit
  • Other Designations:
  • OTTMUSP00000021321
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