Rabbit polyclonal antibody raised against partial recombinant Hif1a.
Recombinant protein corresponding to amino acids 530-825 of mouse Hif1a.
Human, Mouse, Primates, Rat
This antibody is specific to mouse HIF-1 alpha.
Quality Control Testing:
Antibody Reactive Against Recombinant Protein.
Immunocytochemistry (1:100) Immunofluorescence (1:100) Immunohistochemistry (Frozen sections) (5-10 ug/mL) Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (5-10 ug/mL) Western Blot (1:500-1:2000) The optimal working dilution should be determined by the end user.
In PBS (0.05% sodium azide)
Store at 4°C. Do not freeze.
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Western blot analysis of Hif1a in a hypoxic sample using Hif1a polyclonal antibody (Cat # PAB12138). Lane 1 : CoCl2 treated COS-7 nuclear extract (50 ug, hypoxic). Lane 2 : Untreated COS-7 nuclear extract (50 ug, normoxic). 10 second ECL exposure.